Leptin responsiveness in mice that ectopically express agouti protein

Ruth Babette Harris, Tiffany D. Mitchell, Randall L. Mynatt

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Agouti protein is an endogenous antagonist of melanocortin receptors (MCR), including MCR3 and MCR4, which have been implicated as part of the hypothalamic mechanism that mediates leptin-induced hypophagia. In this experiment we examined the effects of peripheral and central leptin administration in male and female β-actin promoter (BAPa) mice that express agouti protein ectopically and have a phenotype that includes obesity and diabetes which is exaggerated in males compared with females. Intraperitoneal infusion of 10 μg leptin/day for 13 days caused weight loss and a transient inhibition of food intake in wild-type mice, with a greater effect in males than females. Male BAPa mice were resistant to leptin infusion whereas female mice lost weight. All of the mice lost body weight following a single intracerebroventricular injection of leptin but the effect was greater in female BAPa mice than any other group. There also was a delayed suppression of food intake that was the same for wild-type and BAPa female mice, whereas food intake recovered faster in BAPa than wild-type males. The dissociation between food intake and body weight loss implies a significant effect of leptin on energy expenditure in BAPa mice. These results demonstrate that the effect of leptin on energy balance is not entirely dependent upon the melanocortin system.

Original languageEnglish (US)
Pages (from-to)159-167
Number of pages9
JournalPhysiology and Behavior
Volume75
Issue number1-2
DOIs
StatePublished - Feb 1 2002
Externally publishedYes

Fingerprint

Agouti Signaling Protein
Leptin
Eating
Weight Loss
Melanocortin Receptors
Body Weight
Parenteral Infusions
Melanocortins
Energy Metabolism
Actins
Obesity
Phenotype
Weights and Measures
Injections

Keywords

  • BAPa mice
  • Gender
  • Thermogenesis
  • Weight loss

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Cite this

Leptin responsiveness in mice that ectopically express agouti protein. / Harris, Ruth Babette; Mitchell, Tiffany D.; Mynatt, Randall L.

In: Physiology and Behavior, Vol. 75, No. 1-2, 01.02.2002, p. 159-167.

Research output: Contribution to journalArticle

Harris, Ruth Babette ; Mitchell, Tiffany D. ; Mynatt, Randall L. / Leptin responsiveness in mice that ectopically express agouti protein. In: Physiology and Behavior. 2002 ; Vol. 75, No. 1-2. pp. 159-167.
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