Lhx9 Is Required for the Development of Retinal Nitric Oxide-Synthesizing Amacrine Cell Subtype

Revathi Balasubramanian, Andrew Bui, Xuhui Dong, Lin Gan

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Amacrine cells are the most diverse group of retinal neurons. Various subtypes of amacrine interneurons mediate a vast majority of image forming and non-image forming visual functions. The transcriptional regulation governing the development of individual amacrine cell subtypes is not well understood. One such amacrine cell subtype comprises neuronal nitric oxide synthase (nNOS/bNOS/NOS1)-expressing amacrine cells (NOACs) that regulate the release of nitric oxide (NO), a neurotransmitter with physiological and clinical implications in the retina. We have identified the LIM-homeodomain transcription factor LHX9 to be necessary for the genesis of NOACs. During retinal development, NOACs express Lhx9, and Lhx9-null retinas lack NOACs. Lhx9-null retinas also display aberrations in dendritic stratification at the inner plexiform layer. Our cell lineage-tracing studies show that Lhx9-expressing cells give rise to both the GAD65 and GAD67 expressing sub-populations of GABAergic amacrine cells. As development proceeds, Lhx9 is downregulated in the GAD65 sub-population of GABAergic cells and is largely restricted to the GAD67 sub-population of amacrine cells that NOACs are a part of. Taken together, we have uncovered Lhx9 as a new molecular marker that defines a subset of amacrine cells and show that it is necessary for the development of the NOAC subtype of amacrine cells.

Original languageEnglish (US)
Pages (from-to)2922-2933
Number of pages12
JournalMolecular Neurobiology
Volume55
Issue number4
DOIs
StatePublished - Apr 1 2018
Externally publishedYes

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Keywords

  • Amacrine cell subtype
  • LIM-homeodomain transcription factor
  • Retina
  • Retinal lamination

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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