Liberation of hydroxyproline from gelatin by acid hydrolysis

David Henry Pashley, C. Keith Claycomb, Gordon W. Summers

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A simple technique is described for the hydrolysis of gelatin resulting in good recovery of hydroxyproline. Hydrolysis of not more than 240 mg of gelatin in 4 ml of approximately 6 N HCl gives maximum yield of hydroxyproline after 12 hr at 100°C, with the required time decreasing to 1 hr at 130-150°.

Original languageEnglish (US)
Pages (from-to)154-156
Number of pages3
JournalAnalytical Biochemistry
Volume15
Issue number1
DOIs
StatePublished - Jan 1 1966

Fingerprint

Hydroxyproline
Gelatin
Hydrolysis
Acids
Recovery

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Liberation of hydroxyproline from gelatin by acid hydrolysis. / Pashley, David Henry; Claycomb, C. Keith; Summers, Gordon W.

In: Analytical Biochemistry, Vol. 15, No. 1, 01.01.1966, p. 154-156.

Research output: Contribution to journalArticle

Pashley, David Henry ; Claycomb, C. Keith ; Summers, Gordon W. / Liberation of hydroxyproline from gelatin by acid hydrolysis. In: Analytical Biochemistry. 1966 ; Vol. 15, No. 1. pp. 154-156.
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