Ligand-bound quantum dot probes for studying the molecular scale dynamics of receptor endocytic trafficking in live cells

Sujata Sundara Rajan, HongYan Liu, Tania Q. Vu

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Endocytic receptor trafficking is a complex, dynamic process underlying fundamental cell function. An integrated understanding of endocytosis at the level of single or small numbers of ligand bound-receptor complexes inside live cells is currently hampered by technical limitations. Here, we develop and test ligand nerve growth factor-bound quantum dot (NGF-QD) bioconjugates for imaging discrete receptor endocytic events inside live NGF-responsive PC12 cells. Using single particle tracking, QD hybrid gel coimmunoprecipitation, and immunocolocalization, we illustrate and validate the use of QD-receptor complexes for imaging receptor trafficking at synchronized time points after QD-ligand - receptor binding and internalization (t = 15-150 min). The unique value of these probes is illustrated by new dynamic observations: (1) that endocytosis proceeds at strikingly regulated fashion, and (2) that diffusive and active forms of transport inside cells are rapid and efficient. QDs are powerful intracellular probes that can provide biologists with new capabilities and fresh insight for studying endocytic receptor signaling events, in real time, and at the resolution of single or small numbers of receptors in live cells.

Original languageEnglish (US)
Pages (from-to)1153-1166
Number of pages14
JournalACS Nano
Volume2
Issue number6
DOIs
StatePublished - Jun 1 2008

Fingerprint

Molecular Probes
Semiconductor quantum dots
Ligands
quantum dots
Nerve Growth Factor
ligands
probes
cells
Imaging techniques
Particles (particulate matter)
Gels
nerves
gels

Keywords

  • Active transport
  • Endocytosis
  • Microtubule
  • Neurotrophin
  • Quantum dot
  • Receptor
  • Trafficking

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Ligand-bound quantum dot probes for studying the molecular scale dynamics of receptor endocytic trafficking in live cells. / Sundara Rajan, Sujata; Liu, HongYan; Vu, Tania Q.

In: ACS Nano, Vol. 2, No. 6, 01.06.2008, p. 1153-1166.

Research output: Contribution to journalArticle

Sundara Rajan, Sujata ; Liu, HongYan ; Vu, Tania Q. / Ligand-bound quantum dot probes for studying the molecular scale dynamics of receptor endocytic trafficking in live cells. In: ACS Nano. 2008 ; Vol. 2, No. 6. pp. 1153-1166.
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