Ligand bound quantum dots for intracellular imaging of neural receptors

Tania Q. Vu, Sujata Sundara Rajan, HongYan Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Quantum dots (QDs) may serve as improved platforms for the complex modulation and ultra-sensitive imaging of molecular signaling in cells. The time course and spatial localization of activated ligand-receptor complexes and their trafficking within cells is becoming increasingly understood as vital for propagating cell signals. However, the movement and fate of ligand-receptor pairs inside cells is difficult to define with current technologies. We have studied the intracellular trafficking of TrkA receptors using QDs conjugated with nerve growth factor, a neuropeptide ligand critical for nervous system development and regulation. We find that NGF-QDs bind and activate TrkA surface receptors in PC12 neurons. Spatiotemporal maps of TrkA-NGF-QD endocytosis and translocation can be directly visualized with single QD resolution. Moreover, single molecule tracking experiments indicates that QDs complexes are actively shuttled over long distances within newly-sprouted neuronal processes. These results indicate that QDs can serve as effective high-resolution probe to track ligand-receptor function in the interior of cells.

Original languageEnglish (US)
Title of host publicationColloidal Quantum Dots for Biomedical Applications II
Volume6448
DOIs
StatePublished - Sep 3 2007
Externally publishedYes
EventColloidal Quantum Dots for Biomedical Applications II - San Jose, CA, United States
Duration: Jan 20 2007Jan 23 2007

Other

OtherColloidal Quantum Dots for Biomedical Applications II
CountryUnited States
CitySan Jose, CA
Period1/20/071/23/07

Fingerprint

Quantum Dots
Sensory Receptor Cells
Semiconductor quantum dots
Ligands
quantum dots
Imaging techniques
ligands
Nerve Growth Factor
trkA Receptor
cells
nervous system
Molecular Imaging
nerves
Neurology
Endocytosis
Neuropeptides
neurons
Nervous System
Neurons
platforms

Keywords

  • Intracellular
  • Ligand
  • Nerve growth factor
  • Neuron
  • Quantum dot
  • Receptor
  • Single molecule
  • Tracking
  • Trafficking
  • TrkA

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Vu, T. Q., Rajan, S. S., & Liu, H. (2007). Ligand bound quantum dots for intracellular imaging of neural receptors. In Colloidal Quantum Dots for Biomedical Applications II (Vol. 6448). [644813] https://doi.org/10.1117/12.724220

Ligand bound quantum dots for intracellular imaging of neural receptors. / Vu, Tania Q.; Rajan, Sujata Sundara; Liu, HongYan.

Colloidal Quantum Dots for Biomedical Applications II. Vol. 6448 2007. 644813.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Vu, TQ, Rajan, SS & Liu, H 2007, Ligand bound quantum dots for intracellular imaging of neural receptors. in Colloidal Quantum Dots for Biomedical Applications II. vol. 6448, 644813, Colloidal Quantum Dots for Biomedical Applications II, San Jose, CA, United States, 1/20/07. https://doi.org/10.1117/12.724220
Vu TQ, Rajan SS, Liu H. Ligand bound quantum dots for intracellular imaging of neural receptors. In Colloidal Quantum Dots for Biomedical Applications II. Vol. 6448. 2007. 644813 https://doi.org/10.1117/12.724220
Vu, Tania Q. ; Rajan, Sujata Sundara ; Liu, HongYan. / Ligand bound quantum dots for intracellular imaging of neural receptors. Colloidal Quantum Dots for Biomedical Applications II. Vol. 6448 2007.
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