Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines

A. J. Miles, Robert W. Janes, A. Brown, D. T. Clarke, J. C. Sutherland, Y. Tao, B. A. Wallace, S. V. Hoffmann

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.

Original languageEnglish (US)
Pages (from-to)420-422
Number of pages3
JournalJournal of Synchrotron Radiation
Volume15
Issue number4
DOIs
StatePublished - Jul 7 2008
Externally publishedYes

Keywords

  • Photon flux
  • Protein denaturation
  • Radiation damage
  • Synchrotron radiation circular dichroism spectroscopy
  • Vacuum ultraviolet wavelengths

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

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