Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines

A. J. Miles, Robert W. Janes, A. Brown, D. T. Clarke, J. C. Sutherland, Y. Tao, B. A. Wallace, S. V. Hoffmann

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.

Original languageEnglish (US)
Pages (from-to)420-422
Number of pages3
JournalJournal of Synchrotron Radiation
Volume15
Issue number4
DOIs
StatePublished - Jul 7 2008
Externally publishedYes

Fingerprint

Denaturation
biopolymer denaturation
Dichroism
Synchrotron radiation
dichroism
synchrotron radiation
flux density
Fluxes
Proteins
thresholds
far ultraviolet radiation
Radiation
Denmark
radiation
biology
Ultraviolet radiation
Vacuum
damage
proteins
Wavelength

Keywords

  • Photon flux
  • Protein denaturation
  • Radiation damage
  • Synchrotron radiation circular dichroism spectroscopy
  • Vacuum ultraviolet wavelengths

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

Cite this

Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines. / Miles, A. J.; Janes, Robert W.; Brown, A.; Clarke, D. T.; Sutherland, J. C.; Tao, Y.; Wallace, B. A.; Hoffmann, S. V.

In: Journal of Synchrotron Radiation, Vol. 15, No. 4, 07.07.2008, p. 420-422.

Research output: Contribution to journalArticle

Miles, A. J. ; Janes, Robert W. ; Brown, A. ; Clarke, D. T. ; Sutherland, J. C. ; Tao, Y. ; Wallace, B. A. ; Hoffmann, S. V. / Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines. In: Journal of Synchrotron Radiation. 2008 ; Vol. 15, No. 4. pp. 420-422.
@article{012d39f2cad34319a67cfecc9bbff4bf,
title = "Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines",
abstract = "New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.",
keywords = "Photon flux, Protein denaturation, Radiation damage, Synchrotron radiation circular dichroism spectroscopy, Vacuum ultraviolet wavelengths",
author = "Miles, {A. J.} and Janes, {Robert W.} and A. Brown and Clarke, {D. T.} and Sutherland, {J. C.} and Y. Tao and Wallace, {B. A.} and Hoffmann, {S. V.}",
year = "2008",
month = "7",
day = "7",
doi = "10.1107/S0909049508009606",
language = "English (US)",
volume = "15",
pages = "420--422",
journal = "Journal of Synchrotron Radiation",
issn = "0909-0495",
publisher = "International Union of Crystallography",
number = "4",

}

TY - JOUR

T1 - Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines

AU - Miles, A. J.

AU - Janes, Robert W.

AU - Brown, A.

AU - Clarke, D. T.

AU - Sutherland, J. C.

AU - Tao, Y.

AU - Wallace, B. A.

AU - Hoffmann, S. V.

PY - 2008/7/7

Y1 - 2008/7/7

N2 - New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.

AB - New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.

KW - Photon flux

KW - Protein denaturation

KW - Radiation damage

KW - Synchrotron radiation circular dichroism spectroscopy

KW - Vacuum ultraviolet wavelengths

UR - http://www.scopus.com/inward/record.url?scp=46149090409&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46149090409&partnerID=8YFLogxK

U2 - 10.1107/S0909049508009606

DO - 10.1107/S0909049508009606

M3 - Article

C2 - 18552437

AN - SCOPUS:46149090409

VL - 15

SP - 420

EP - 422

JO - Journal of Synchrotron Radiation

JF - Journal of Synchrotron Radiation

SN - 0909-0495

IS - 4

ER -