LINKED‐FUNCTION ANALYSIS OF FLUORESCENCE DECAY KINETICS: RESOLUTION OF SIDE‐CHAIN ROTAMER POPULATIONS OF A SINGLE AROMATIC AMINO ACID IN SMALL POLYPEPTIDES

J. B. Alexander Ross, William R. Laws, John Clark Sutherland, Angeliki Buku, Panayotis G. Katsoyannis, Irving L. Schwartz, Herman R. Wyssbrod

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22 Scopus citations

Abstract

A linked‐function approach to fluorescence decay data analysis is presented that permits complex systems to be resolved from a single decay curve. The method involves linking fluorescence decay parameters based on a relationship established by independent physical measurements. As an example, by correlating the fluorescence data with 1H‐NMR results, the complex fluorescence decay kinetics of tyrosine analogs and single tyrosyl residues in simple polypeptides can be explained by ground‐state rotameric populations of the phenol ring about the Cα‐Cβ bond.

Original languageEnglish (US)
Pages (from-to)365-370
Number of pages6
JournalPhotochemistry and Photobiology
Volume44
Issue number3
DOIs
Publication statusPublished - Sep 1986
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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