LINKED‐FUNCTION ANALYSIS OF FLUORESCENCE DECAY KINETICS

RESOLUTION OF SIDE‐CHAIN ROTAMER POPULATIONS OF A SINGLE AROMATIC AMINO ACID IN SMALL POLYPEPTIDES

J. B. Alexander Ross, William R. Laws, John C. Sutherland, Angeliki Buku, Panayotis G. Katsoyannis, Irving L. Schwartz, Herman R. Wyssbrod

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A linked‐function approach to fluorescence decay data analysis is presented that permits complex systems to be resolved from a single decay curve. The method involves linking fluorescence decay parameters based on a relationship established by independent physical measurements. As an example, by correlating the fluorescence data with 1H‐NMR results, the complex fluorescence decay kinetics of tyrosine analogs and single tyrosyl residues in simple polypeptides can be explained by ground‐state rotameric populations of the phenol ring about the Cα‐Cβ bond.

Original languageEnglish (US)
Pages (from-to)365-370
Number of pages6
JournalPhotochemistry and Photobiology
Volume44
Issue number3
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Aromatic Amino Acids
polypeptides
amino acids
Fluorescence
fluorescence
Peptides
Kinetics
kinetics
decay
Population
tyrosine
Phenol
complex systems
phenols
Tyrosine
Large scale systems
analogs
rings
curves

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

LINKED‐FUNCTION ANALYSIS OF FLUORESCENCE DECAY KINETICS : RESOLUTION OF SIDE‐CHAIN ROTAMER POPULATIONS OF A SINGLE AROMATIC AMINO ACID IN SMALL POLYPEPTIDES. / Alexander Ross, J. B.; Laws, William R.; Sutherland, John C.; Buku, Angeliki; Katsoyannis, Panayotis G.; Schwartz, Irving L.; Wyssbrod, Herman R.

In: Photochemistry and Photobiology, Vol. 44, No. 3, 01.01.1986, p. 365-370.

Research output: Contribution to journalArticle

Alexander Ross, J. B. ; Laws, William R. ; Sutherland, John C. ; Buku, Angeliki ; Katsoyannis, Panayotis G. ; Schwartz, Irving L. ; Wyssbrod, Herman R. / LINKED‐FUNCTION ANALYSIS OF FLUORESCENCE DECAY KINETICS : RESOLUTION OF SIDE‐CHAIN ROTAMER POPULATIONS OF A SINGLE AROMATIC AMINO ACID IN SMALL POLYPEPTIDES. In: Photochemistry and Photobiology. 1986 ; Vol. 44, No. 3. pp. 365-370.
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abstract = "A linked‐function approach to fluorescence decay data analysis is presented that permits complex systems to be resolved from a single decay curve. The method involves linking fluorescence decay parameters based on a relationship established by independent physical measurements. As an example, by correlating the fluorescence data with 1H‐NMR results, the complex fluorescence decay kinetics of tyrosine analogs and single tyrosyl residues in simple polypeptides can be explained by ground‐state rotameric populations of the phenol ring about the Cα‐Cβ bond.",
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