TY - JOUR
T1 - LINKED‐FUNCTION ANALYSIS OF FLUORESCENCE DECAY KINETICS
T2 - RESOLUTION OF SIDE‐CHAIN ROTAMER POPULATIONS OF A SINGLE AROMATIC AMINO ACID IN SMALL POLYPEPTIDES
AU - Alexander Ross, J. B.
AU - Laws, William R.
AU - Sutherland, John Clark
AU - Buku, Angeliki
AU - Katsoyannis, Panayotis G.
AU - Schwartz, Irving L.
AU - Wyssbrod, Herman R.
PY - 1986/9
Y1 - 1986/9
N2 - A linked‐function approach to fluorescence decay data analysis is presented that permits complex systems to be resolved from a single decay curve. The method involves linking fluorescence decay parameters based on a relationship established by independent physical measurements. As an example, by correlating the fluorescence data with 1H‐NMR results, the complex fluorescence decay kinetics of tyrosine analogs and single tyrosyl residues in simple polypeptides can be explained by ground‐state rotameric populations of the phenol ring about the Cα‐Cβ bond.
AB - A linked‐function approach to fluorescence decay data analysis is presented that permits complex systems to be resolved from a single decay curve. The method involves linking fluorescence decay parameters based on a relationship established by independent physical measurements. As an example, by correlating the fluorescence data with 1H‐NMR results, the complex fluorescence decay kinetics of tyrosine analogs and single tyrosyl residues in simple polypeptides can be explained by ground‐state rotameric populations of the phenol ring about the Cα‐Cβ bond.
UR - http://www.scopus.com/inward/record.url?scp=0022774512&partnerID=8YFLogxK
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U2 - 10.1111/j.1751-1097.1986.tb04677.x
DO - 10.1111/j.1751-1097.1986.tb04677.x
M3 - Article
C2 - 3786458
AN - SCOPUS:0022774512
SN - 0031-8655
VL - 44
SP - 365
EP - 370
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 3
ER -