A linked‐function approach to fluorescence decay data analysis is presented that permits complex systems to be resolved from a single decay curve. The method involves linking fluorescence decay parameters based on a relationship established by independent physical measurements. As an example, by correlating the fluorescence data with 1H‐NMR results, the complex fluorescence decay kinetics of tyrosine analogs and single tyrosyl residues in simple polypeptides can be explained by ground‐state rotameric populations of the phenol ring about the Cα‐Cβ bond.
|Original language||English (US)|
|Number of pages||6|
|Journal||Photochemistry and Photobiology|
|Publication status||Published - Sep 1986|
ASJC Scopus subject areas
- Physical and Theoretical Chemistry