Linking electrophoretic resolution with experimental conditions

John C. Sutherland

doi:10.1117/12.274367

Linking electrophoretic resolution with experimental conditions

Research output: Contribution to journal › Conference article › peer-review

Abstract

The effects of gel composition and electric field strength on electrophoretic resolving power were reported. The relationship between parameters describing DNA mobility as a function of molecular length was described for systematic variations of gel composition and electric field strength. These parameters were related to experimentally controllable factors such as the density of the gel matrix and the strength of the electric field. The results showed that the longer DNAs were best separated using low density gel matrices and low electric fields.

Original language	English (US)
Pages (from-to)	47-60
Number of pages	14
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	2985
DOIs	https://doi.org/10.1117/12.274367
State	Published - Dec 1 1997
Externally published	Yes
Event	Ultrasensitive Biochemical Diagnostics II - San Jose, CA, United States Duration: Feb 10 1997 → Feb 12 1997

Keywords

Agarose gel electrophoresis
Electrophoretic resolution
Resolving power
Separation of DNA by static field gel electrophoresis

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.274367

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title = "Linking electrophoretic resolution with experimental conditions",

abstract = "The effects of gel composition and electric field strength on electrophoretic resolving power were reported. The relationship between parameters describing DNA mobility as a function of molecular length was described for systematic variations of gel composition and electric field strength. These parameters were related to experimentally controllable factors such as the density of the gel matrix and the strength of the electric field. The results showed that the longer DNAs were best separated using low density gel matrices and low electric fields.",

keywords = "Agarose gel electrophoresis, Electrophoretic resolution, Resolving power, Separation of DNA by static field gel electrophoresis",

author = "Sutherland, {John C.}",

year = "1997",

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doi = "10.1117/12.274367",

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volume = "2985",

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T1 - Linking electrophoretic resolution with experimental conditions

AU - Sutherland, John C.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - The effects of gel composition and electric field strength on electrophoretic resolving power were reported. The relationship between parameters describing DNA mobility as a function of molecular length was described for systematic variations of gel composition and electric field strength. These parameters were related to experimentally controllable factors such as the density of the gel matrix and the strength of the electric field. The results showed that the longer DNAs were best separated using low density gel matrices and low electric fields.

AB - The effects of gel composition and electric field strength on electrophoretic resolving power were reported. The relationship between parameters describing DNA mobility as a function of molecular length was described for systematic variations of gel composition and electric field strength. These parameters were related to experimentally controllable factors such as the density of the gel matrix and the strength of the electric field. The results showed that the longer DNAs were best separated using low density gel matrices and low electric fields.

KW - Agarose gel electrophoresis

KW - Electrophoretic resolution

KW - Resolving power

KW - Separation of DNA by static field gel electrophoresis

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DO - 10.1117/12.274367

M3 - Conference article

AN - SCOPUS:0031389721

SN - 0277-786X

VL - 2985

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EP - 60

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Ultrasensitive Biochemical Diagnostics II

Y2 - 10 February 1997 through 12 February 1997

ER -

Linking electrophoretic resolution with experimental conditions

Abstract

Keywords

ASJC Scopus subject areas

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