Linking electrophoretic resolution with experimental conditions

Research output: Contribution to journalConference article

Abstract

The effects of gel composition and electric field strength on electrophoretic resolving power were reported. The relationship between parameters describing DNA mobility as a function of molecular length was described for systematic variations of gel composition and electric field strength. These parameters were related to experimentally controllable factors such as the density of the gel matrix and the strength of the electric field. The results showed that the longer DNAs were best separated using low density gel matrices and low electric fields.

Original languageEnglish (US)
Pages (from-to)47-60
Number of pages14
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume2985
DOIs
StatePublished - Dec 1 1997
EventUltrasensitive Biochemical Diagnostics II - San Jose, CA, United States
Duration: Feb 10 1997Feb 12 1997

Fingerprint

Linking
Electric Field
Gels
Electric fields
gels
electric field strength
DNA
deoxyribonucleic acid
electric fields
Optical resolving power
matrices
Chemical analysis

Keywords

  • Agarose gel electrophoresis
  • Electrophoretic resolution
  • Resolving power
  • Separation of DNA by static field gel electrophoresis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Linking electrophoretic resolution with experimental conditions. / Sutherland, John C.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 2985, 01.12.1997, p. 47-60.

Research output: Contribution to journalConference article

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