Lipid and Protein Alterations of Spinal Cord and Cord Myelin of Multiple Sclerosis

Robert K Yu, Kunihiro Ueno, Gilbert H. Glaser, Wallace W. Tourtellotte

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Abstract: A comprehensive study was carried out to clarify the chemical compositions of spinal cord, cord myelin, and myelin subfractions of multiple sclerosis (MS). The protein compositions of normal‐appearing cerebral white matter and cerebral plaque and periplaque tissues were also analyzed for comparison. MS whole cord samples were found to contain higher amounts of water compared with normal samples. The total lipid contents were below normal. Among the individual lipids, cholesterol content remained unchanged, whereas cholesteryl esters appeared increased in MS cords. The acidic phospholipid concentrations were found to be lower than normal. Glycolipids, such as cerebrosides GM4, GM1, and GD1b, which are abundant in myelin, were all decreased. However, the concentrations of GM3 and GD3, which are more characteristic of reactive astrocytes, were highly elevated. The total protein content of MS cord samples was decreased, and the decrease was attributable to the loss of myelin proteins as evidenced by the low recovery of myelin. The concentrations of myelin‐specific proteins, such as proteolipid protein and myelin basic protein, were significantly reduced. Other changes in the protein compositions included the accretion of two low molecular weight proteins of approximately 11,000 and 12,000, and the appearance of a periodic acid‐Schiff‐positive protein with the same electrophoretic mobility as the P0 protein. Analysis of the isolated myelin indicated that it had a grossly normal protein composition. However, the two low molecular weight proteins and the P0 protein appeared to be enriched in an upper‐phase cord subtraction. We attribute the appearance of the two low molecular weight proteins to the breakdown of proteolipid protein and/or myelin basic protein as a result of demyelination, and the appearance of P0 to the involvement of PNS myelin. The latter finding provides the first biochemical evidence that in MS cord, remyelination can be achieved in part by invading Schwann cells and/or by the small number of Schwann cells that may be present in the cord.

Original languageEnglish (US)
Pages (from-to)464-477
Number of pages14
JournalJournal of Neurochemistry
Volume39
Issue number2
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

Myelin Sheath
Multiple Sclerosis
Spinal Cord
Lipids
Proteins
Myelin P0 Protein
Proteolipids
Myelin Basic Protein
Molecular Weight
Molecular weight
Schwann Cells
Chemical analysis
Cells
Cerebrosides
Myelin Proteins
Electrophoretic mobility
Cholesterol Esters
Glycolipids
Demyelinating Diseases
Astrocytes

Keywords

  • Central nervous system
  • Gangliosides
  • Lipids
  • Multiple sclerosis
  • Myelin
  • Proteins
  • Spinal cord

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Lipid and Protein Alterations of Spinal Cord and Cord Myelin of Multiple Sclerosis. / Yu, Robert K; Ueno, Kunihiro; Glaser, Gilbert H.; Tourtellotte, Wallace W.

In: Journal of Neurochemistry, Vol. 39, No. 2, 01.01.1982, p. 464-477.

Research output: Contribution to journalArticle

Yu, Robert K ; Ueno, Kunihiro ; Glaser, Gilbert H. ; Tourtellotte, Wallace W. / Lipid and Protein Alterations of Spinal Cord and Cord Myelin of Multiple Sclerosis. In: Journal of Neurochemistry. 1982 ; Vol. 39, No. 2. pp. 464-477.
@article{eb227dbb90614025a5663158d1194c13,
title = "Lipid and Protein Alterations of Spinal Cord and Cord Myelin of Multiple Sclerosis",
abstract = "Abstract: A comprehensive study was carried out to clarify the chemical compositions of spinal cord, cord myelin, and myelin subfractions of multiple sclerosis (MS). The protein compositions of normal‐appearing cerebral white matter and cerebral plaque and periplaque tissues were also analyzed for comparison. MS whole cord samples were found to contain higher amounts of water compared with normal samples. The total lipid contents were below normal. Among the individual lipids, cholesterol content remained unchanged, whereas cholesteryl esters appeared increased in MS cords. The acidic phospholipid concentrations were found to be lower than normal. Glycolipids, such as cerebrosides GM4, GM1, and GD1b, which are abundant in myelin, were all decreased. However, the concentrations of GM3 and GD3, which are more characteristic of reactive astrocytes, were highly elevated. The total protein content of MS cord samples was decreased, and the decrease was attributable to the loss of myelin proteins as evidenced by the low recovery of myelin. The concentrations of myelin‐specific proteins, such as proteolipid protein and myelin basic protein, were significantly reduced. Other changes in the protein compositions included the accretion of two low molecular weight proteins of approximately 11,000 and 12,000, and the appearance of a periodic acid‐Schiff‐positive protein with the same electrophoretic mobility as the P0 protein. Analysis of the isolated myelin indicated that it had a grossly normal protein composition. However, the two low molecular weight proteins and the P0 protein appeared to be enriched in an upper‐phase cord subtraction. We attribute the appearance of the two low molecular weight proteins to the breakdown of proteolipid protein and/or myelin basic protein as a result of demyelination, and the appearance of P0 to the involvement of PNS myelin. The latter finding provides the first biochemical evidence that in MS cord, remyelination can be achieved in part by invading Schwann cells and/or by the small number of Schwann cells that may be present in the cord.",
keywords = "Central nervous system, Gangliosides, Lipids, Multiple sclerosis, Myelin, Proteins, Spinal cord",
author = "Yu, {Robert K} and Kunihiro Ueno and Glaser, {Gilbert H.} and Tourtellotte, {Wallace W.}",
year = "1982",
month = "1",
day = "1",
doi = "10.1111/j.1471-4159.1982.tb03968.x",
language = "English (US)",
volume = "39",
pages = "464--477",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Lipid and Protein Alterations of Spinal Cord and Cord Myelin of Multiple Sclerosis

AU - Yu, Robert K

AU - Ueno, Kunihiro

AU - Glaser, Gilbert H.

AU - Tourtellotte, Wallace W.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - Abstract: A comprehensive study was carried out to clarify the chemical compositions of spinal cord, cord myelin, and myelin subfractions of multiple sclerosis (MS). The protein compositions of normal‐appearing cerebral white matter and cerebral plaque and periplaque tissues were also analyzed for comparison. MS whole cord samples were found to contain higher amounts of water compared with normal samples. The total lipid contents were below normal. Among the individual lipids, cholesterol content remained unchanged, whereas cholesteryl esters appeared increased in MS cords. The acidic phospholipid concentrations were found to be lower than normal. Glycolipids, such as cerebrosides GM4, GM1, and GD1b, which are abundant in myelin, were all decreased. However, the concentrations of GM3 and GD3, which are more characteristic of reactive astrocytes, were highly elevated. The total protein content of MS cord samples was decreased, and the decrease was attributable to the loss of myelin proteins as evidenced by the low recovery of myelin. The concentrations of myelin‐specific proteins, such as proteolipid protein and myelin basic protein, were significantly reduced. Other changes in the protein compositions included the accretion of two low molecular weight proteins of approximately 11,000 and 12,000, and the appearance of a periodic acid‐Schiff‐positive protein with the same electrophoretic mobility as the P0 protein. Analysis of the isolated myelin indicated that it had a grossly normal protein composition. However, the two low molecular weight proteins and the P0 protein appeared to be enriched in an upper‐phase cord subtraction. We attribute the appearance of the two low molecular weight proteins to the breakdown of proteolipid protein and/or myelin basic protein as a result of demyelination, and the appearance of P0 to the involvement of PNS myelin. The latter finding provides the first biochemical evidence that in MS cord, remyelination can be achieved in part by invading Schwann cells and/or by the small number of Schwann cells that may be present in the cord.

AB - Abstract: A comprehensive study was carried out to clarify the chemical compositions of spinal cord, cord myelin, and myelin subfractions of multiple sclerosis (MS). The protein compositions of normal‐appearing cerebral white matter and cerebral plaque and periplaque tissues were also analyzed for comparison. MS whole cord samples were found to contain higher amounts of water compared with normal samples. The total lipid contents were below normal. Among the individual lipids, cholesterol content remained unchanged, whereas cholesteryl esters appeared increased in MS cords. The acidic phospholipid concentrations were found to be lower than normal. Glycolipids, such as cerebrosides GM4, GM1, and GD1b, which are abundant in myelin, were all decreased. However, the concentrations of GM3 and GD3, which are more characteristic of reactive astrocytes, were highly elevated. The total protein content of MS cord samples was decreased, and the decrease was attributable to the loss of myelin proteins as evidenced by the low recovery of myelin. The concentrations of myelin‐specific proteins, such as proteolipid protein and myelin basic protein, were significantly reduced. Other changes in the protein compositions included the accretion of two low molecular weight proteins of approximately 11,000 and 12,000, and the appearance of a periodic acid‐Schiff‐positive protein with the same electrophoretic mobility as the P0 protein. Analysis of the isolated myelin indicated that it had a grossly normal protein composition. However, the two low molecular weight proteins and the P0 protein appeared to be enriched in an upper‐phase cord subtraction. We attribute the appearance of the two low molecular weight proteins to the breakdown of proteolipid protein and/or myelin basic protein as a result of demyelination, and the appearance of P0 to the involvement of PNS myelin. The latter finding provides the first biochemical evidence that in MS cord, remyelination can be achieved in part by invading Schwann cells and/or by the small number of Schwann cells that may be present in the cord.

KW - Central nervous system

KW - Gangliosides

KW - Lipids

KW - Multiple sclerosis

KW - Myelin

KW - Proteins

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0020265887&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020265887&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1982.tb03968.x

DO - 10.1111/j.1471-4159.1982.tb03968.x

M3 - Article

VL - 39

SP - 464

EP - 477

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 2

ER -