Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA

Ruslan Rafikov, Christiana Dimitropoulou, Saurabh Aggarwal, Archana Kangath, Christine Gross, Daniel Pardo, Shruti Sharma, Agnieszka Jezierska-Drutel, Vijaykumar Surendrakant Patel, Connie Snead, Rudolf Lucas, Alexander Dmitriyevich Verin, David J Fulton, John D. Catravas, Stephen Matthew Black

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: The activation of RhoA is a critical event in acute lung injury (ALI), but the role of nitration in this process is unresolved. Results: The nitration of RhoA at Tyr34 produced GEF-like conformational changes that stimulate RhoA by decreasing GDP binding. Conclusion: We have identified a new mechanism of RhoA activation. Significance: Preventing RhoA nitration may be useful for the management of ALI.

Original languageEnglish (US)
Pages (from-to)4710-4722
Number of pages13
JournalJournal of Biological Chemistry
Volume289
Issue number8
DOIs
StatePublished - Feb 21 2014

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Nitration
Acute Lung Injury
Lung Injury
Lipopolysaccharides
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Rafikov, R., Dimitropoulou, C., Aggarwal, S., Kangath, A., Gross, C., Pardo, D., ... Black, S. M. (2014). Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. Journal of Biological Chemistry, 289(8), 4710-4722. https://doi.org/10.1074/jbc.M114.547596

Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. / Rafikov, Ruslan; Dimitropoulou, Christiana; Aggarwal, Saurabh; Kangath, Archana; Gross, Christine; Pardo, Daniel; Sharma, Shruti; Jezierska-Drutel, Agnieszka; Patel, Vijaykumar Surendrakant; Snead, Connie; Lucas, Rudolf; Verin, Alexander Dmitriyevich; Fulton, David J; Catravas, John D.; Black, Stephen Matthew.

In: Journal of Biological Chemistry, Vol. 289, No. 8, 21.02.2014, p. 4710-4722.

Research output: Contribution to journalArticle

Rafikov, R, Dimitropoulou, C, Aggarwal, S, Kangath, A, Gross, C, Pardo, D, Sharma, S, Jezierska-Drutel, A, Patel, VS, Snead, C, Lucas, R, Verin, AD, Fulton, DJ, Catravas, JD & Black, SM 2014, 'Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA', Journal of Biological Chemistry, vol. 289, no. 8, pp. 4710-4722. https://doi.org/10.1074/jbc.M114.547596
Rafikov R, Dimitropoulou C, Aggarwal S, Kangath A, Gross C, Pardo D et al. Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. Journal of Biological Chemistry. 2014 Feb 21;289(8):4710-4722. https://doi.org/10.1074/jbc.M114.547596
Rafikov, Ruslan ; Dimitropoulou, Christiana ; Aggarwal, Saurabh ; Kangath, Archana ; Gross, Christine ; Pardo, Daniel ; Sharma, Shruti ; Jezierska-Drutel, Agnieszka ; Patel, Vijaykumar Surendrakant ; Snead, Connie ; Lucas, Rudolf ; Verin, Alexander Dmitriyevich ; Fulton, David J ; Catravas, John D. ; Black, Stephen Matthew. / Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 8. pp. 4710-4722.
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