Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA

Ruslan Rafikov; Christiana Dimitropoulou; Saurabh Aggarwal; Archana Kangath; Christine Gross; Daniel Pardo; Shruti Sharma; Agnieszka Jezierska-Drutel; Vijay Patel; Connie Snead; Rudolf Lucas; Alexander Verin; David Fulton; John D. Catravas; Stephen M. Black

doi:10.1074/jbc.M114.547596

Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA

Ruslan Rafikov, Christiana Dimitropoulou, Saurabh Aggarwal, Archana Kangath, Christine Gross, Daniel Pardo, Shruti Sharma, Agnieszka Jezierska-Drutel, Vijay Patel, Connie Snead, Rudolf Lucas, Alexander Verin, David Fulton, John D. Catravas, Stephen M. Black

Research output: Contribution to journal › Article

29 Scopus citations

Abstract

Background: The activation of RhoA is a critical event in acute lung injury (ALI), but the role of nitration in this process is unresolved. Results: The nitration of RhoA at Tyr³⁴ produced GEF-like conformational changes that stimulate RhoA by decreasing GDP binding. Conclusion: We have identified a new mechanism of RhoA activation. Significance: Preventing RhoA nitration may be useful for the management of ALI.

Original language	English (US)
Pages (from-to)	4710-4722
Number of pages	13
Journal	Journal of Biological Chemistry
Volume	289
Issue number	8
DOIs	https://doi.org/10.1074/jbc.M114.547596
State	Published - Feb 21 2014

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ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Cite this

Rafikov, R., Dimitropoulou, C., Aggarwal, S., Kangath, A., Gross, C., Pardo, D., Sharma, S., Jezierska-Drutel, A., Patel, V., Snead, C., Lucas, R., Verin, A., Fulton, D., Catravas, J. D., & Black, S. M. (2014). Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. Journal of Biological Chemistry, 289(8), 4710-4722. https://doi.org/10.1074/jbc.M114.547596

Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. / Rafikov, Ruslan; Dimitropoulou, Christiana; Aggarwal, Saurabh; Kangath, Archana; Gross, Christine; Pardo, Daniel; Sharma, Shruti; Jezierska-Drutel, Agnieszka; Patel, Vijay; Snead, Connie; Lucas, Rudolf ; Verin, Alexander ; Fulton, David; Catravas, John D.; Black, Stephen M.

In: Journal of Biological Chemistry, Vol. 289, No. 8, 21.02.2014, p. 4710-4722.

Research output: Contribution to journal › Article

Rafikov, R, Dimitropoulou, C, Aggarwal, S, Kangath, A, Gross, C, Pardo, D, Sharma, S, Jezierska-Drutel, A, Patel, V, Snead, C, Lucas, R , Verin, A , Fulton, D, Catravas, JD & Black, SM 2014, 'Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA', Journal of Biological Chemistry, vol. 289, no. 8, pp. 4710-4722. https://doi.org/10.1074/jbc.M114.547596

Rafikov, Ruslan ; Dimitropoulou, Christiana ; Aggarwal, Saurabh ; Kangath, Archana ; Gross, Christine ; Pardo, Daniel ; Sharma, Shruti ; Jezierska-Drutel, Agnieszka ; Patel, Vijay ; Snead, Connie ; Lucas, Rudolf ; Verin, Alexander ; Fulton, David ; Catravas, John D. ; Black, Stephen M. / Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 8. pp. 4710-4722.

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abstract = "Background: The activation of RhoA is a critical event in acute lung injury (ALI), but the role of nitration in this process is unresolved. Results: The nitration of RhoA at Tyr34 produced GEF-like conformational changes that stimulate RhoA by decreasing GDP binding. Conclusion: We have identified a new mechanism of RhoA activation. Significance: Preventing RhoA nitration may be useful for the management of ALI.",

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10.1074/jbc.M114.547596