Liquid chromatographic determination of amino acids and peptides by pre-column fluorescence derivatization with N-hydroxysuccinimidyl-α-(9-acridine)-acetate

Jinmao You, Xingjun Fan, Qingcun Zhu, Yuzhen Su

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A sensitive liquid chromatographic method for the detection of amino acids and peptides with pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, triglycine, glutathione (GSH), glutamic acid, and cysteine were separated on a reversed-phase C18 column. At the same time, 15 representative amino acids were also separated with a gradient program. Derivatization and the chromatographic conditions were optimized in experiments. The derivatives were eluted with a good reproducibility. The linear range of the calibration graph was 0.08-260nmolml-1. The relative standard deviations (n=6) are 4.5%. Detection limits (signal-to-noise ratio=3) for derivatives are at the level of fmol. The methods described are suitable for the analysis of amino acids and peptides in various biological samples. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)69-79
Number of pages11
JournalAnalytica Chimica Acta
Volume367
Issue number1-3
DOIs
StatePublished - Jul 3 1998

Fingerprint

Acridines
peptide
acetate
Acetates
fluorescence
amino acid
Fluorescence
Amino Acids
glycyl-glycyl-glycine
Peptides
liquid
Liquids
Glycylglycine
Derivatives
Signal-To-Noise Ratio
Glycine
signal-to-noise ratio
Calibration
Glutathione
Cysteine

Keywords

  • Amino acids and peptides
  • Column liquid chromatography
  • Fluorescence derivatization
  • N-hydroxysuccinimidyl-α-(9-acridine)-acetate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Liquid chromatographic determination of amino acids and peptides by pre-column fluorescence derivatization with N-hydroxysuccinimidyl-α-(9-acridine)-acetate. / You, Jinmao; Fan, Xingjun; Zhu, Qingcun; Su, Yuzhen.

In: Analytica Chimica Acta, Vol. 367, No. 1-3, 03.07.1998, p. 69-79.

Research output: Contribution to journalArticle

@article{7e95d4beeb1d4be498f3353ea1ac0cf2,
title = "Liquid chromatographic determination of amino acids and peptides by pre-column fluorescence derivatization with N-hydroxysuccinimidyl-α-(9-acridine)-acetate",
abstract = "A sensitive liquid chromatographic method for the detection of amino acids and peptides with pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, triglycine, glutathione (GSH), glutamic acid, and cysteine were separated on a reversed-phase C18 column. At the same time, 15 representative amino acids were also separated with a gradient program. Derivatization and the chromatographic conditions were optimized in experiments. The derivatives were eluted with a good reproducibility. The linear range of the calibration graph was 0.08-260nmolml-1. The relative standard deviations (n=6) are 4.5{\%}. Detection limits (signal-to-noise ratio=3) for derivatives are at the level of fmol. The methods described are suitable for the analysis of amino acids and peptides in various biological samples. Copyright (C) 1998 Elsevier Science B.V.",
keywords = "Amino acids and peptides, Column liquid chromatography, Fluorescence derivatization, N-hydroxysuccinimidyl-α-(9-acridine)-acetate",
author = "Jinmao You and Xingjun Fan and Qingcun Zhu and Yuzhen Su",
year = "1998",
month = "7",
day = "3",
doi = "10.1016/S0003-2670(98)00181-0",
language = "English (US)",
volume = "367",
pages = "69--79",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Liquid chromatographic determination of amino acids and peptides by pre-column fluorescence derivatization with N-hydroxysuccinimidyl-α-(9-acridine)-acetate

AU - You, Jinmao

AU - Fan, Xingjun

AU - Zhu, Qingcun

AU - Su, Yuzhen

PY - 1998/7/3

Y1 - 1998/7/3

N2 - A sensitive liquid chromatographic method for the detection of amino acids and peptides with pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, triglycine, glutathione (GSH), glutamic acid, and cysteine were separated on a reversed-phase C18 column. At the same time, 15 representative amino acids were also separated with a gradient program. Derivatization and the chromatographic conditions were optimized in experiments. The derivatives were eluted with a good reproducibility. The linear range of the calibration graph was 0.08-260nmolml-1. The relative standard deviations (n=6) are 4.5%. Detection limits (signal-to-noise ratio=3) for derivatives are at the level of fmol. The methods described are suitable for the analysis of amino acids and peptides in various biological samples. Copyright (C) 1998 Elsevier Science B.V.

AB - A sensitive liquid chromatographic method for the detection of amino acids and peptides with pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, triglycine, glutathione (GSH), glutamic acid, and cysteine were separated on a reversed-phase C18 column. At the same time, 15 representative amino acids were also separated with a gradient program. Derivatization and the chromatographic conditions were optimized in experiments. The derivatives were eluted with a good reproducibility. The linear range of the calibration graph was 0.08-260nmolml-1. The relative standard deviations (n=6) are 4.5%. Detection limits (signal-to-noise ratio=3) for derivatives are at the level of fmol. The methods described are suitable for the analysis of amino acids and peptides in various biological samples. Copyright (C) 1998 Elsevier Science B.V.

KW - Amino acids and peptides

KW - Column liquid chromatography

KW - Fluorescence derivatization

KW - N-hydroxysuccinimidyl-α-(9-acridine)-acetate

UR - http://www.scopus.com/inward/record.url?scp=0032479121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032479121&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(98)00181-0

DO - 10.1016/S0003-2670(98)00181-0

M3 - Article

AN - SCOPUS:0032479121

VL - 367

SP - 69

EP - 79

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1-3

ER -