Liquid chromatographic determination of amino acids and peptides by pre-column fluorescence derivatization with N-hydroxysuccinimidyl-α-(9-acridine)-acetate

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Abstract

A sensitive liquid chromatographic method for the detection of amino acids and peptides with pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, triglycine, glutathione (GSH), glutamic acid, and cysteine were separated on a reversed-phase C18 column. At the same time, 15 representative amino acids were also separated with a gradient program. Derivatization and the chromatographic conditions were optimized in experiments. The derivatives were eluted with a good reproducibility. The linear range of the calibration graph was 0.08-260nmolml-1. The relative standard deviations (n=6) are 4.5%. Detection limits (signal-to-noise ratio=3) for derivatives are at the level of fmol. The methods described are suitable for the analysis of amino acids and peptides in various biological samples. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)69-79
Number of pages11
JournalAnalytica Chimica Acta
Volume367
Issue number1-3
DOIs
Publication statusPublished - Jul 3 1998
Externally publishedYes

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Keywords

  • Amino acids and peptides
  • Column liquid chromatography
  • Fluorescence derivatization
  • N-hydroxysuccinimidyl-α-(9-acridine)-acetate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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