Liquid chromatography-tandem mass spectrometry analysis of human adrenal vein 19-carbon steroids before and after ACTH stimulation

Juilee Rege, Yasuhiro Nakamura, Fumitoshi Satoh, Ryo Morimoto, Michael R. Kennedy, Lawrence C. Layman, Seijiro Honma, Hironobu Sasano, William E. Rainey

Research output: Contribution to journalArticle

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Abstract

Context: Abroad analysis of adrenal gland-derived 19-carbon (C 19) steroids has not been reported. This is the first study that uses liquid chromatography-tandem mass spectrometry to quantify 9 C19 steroids (androgens and their precursors), estrone, and estradiol in the adrenal vein (AV) of women, before and after ACTH stimulation. Objective: The objective of this study was to define the adrenal androgen metabolome in women before and after ACTH infusion. Design: This was a retrospective study. Patients: Seven women, aged 50.4±5.4 years, with suspected diagnosis of an adrenal aldosterone-producing adenoma were included in the study. Methods: AV and iliac serum samples were collected before and after administration of ACTH (15 minutes). AV samples were analyzed using for concentrations of 9 unconjugated C19 steroids, estrone, and estradiol. Dehydroepiandrosterone sulfate (DHEA-S) was quantified by radioimmunoassay. Results: AV levels of DHEA-S were the highest among the steroids measured. The most abundant unconjugated C 19 steroids in AV were 11β-hydroxyandrostenedione (11OHA), dehydroepiandrosterone (DHEA), and androstenedione (A4). ACTH significantly increased the adrenal output of 9 of the 12 steroids that were measured. ACTH increased the mean AV concentration of DHEA-S by 5-fold, DHEA by 21-fold, A4 by 7-fold, and 11OHA by 5-fold. 11β-Hydroxytestosterone and testosterone were found to be potent androgen receptor agonists when tested with an androgen-responsive cell reporter model. Conclusion: The current study indicates that the adrenal gland secretes primarily 3 weak androgens, namely DHEA, 11OHA, and A4. Active androgens, including testosterone and 11β- hydroxytestosterone, are also produced but to a lesser degree.

Original languageEnglish (US)
Pages (from-to)1182-1188
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume98
Issue number3
DOIs
StatePublished - Mar 1 2013

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Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Adrenocorticotropic Hormone
Androgens
Mass spectrometry
Veins
Carbon
Steroids
Dehydroepiandrosterone Sulfate
Dehydroepiandrosterone
Estrone
Adrenal Glands
Testosterone
Estradiol
Iliac Vein
Metabolome
Androstenedione
Aldosterone
Adenoma

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Liquid chromatography-tandem mass spectrometry analysis of human adrenal vein 19-carbon steroids before and after ACTH stimulation. / Rege, Juilee; Nakamura, Yasuhiro; Satoh, Fumitoshi; Morimoto, Ryo; Kennedy, Michael R.; Layman, Lawrence C.; Honma, Seijiro; Sasano, Hironobu; Rainey, William E.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 98, No. 3, 01.03.2013, p. 1182-1188.

Research output: Contribution to journalArticle

Rege, Juilee ; Nakamura, Yasuhiro ; Satoh, Fumitoshi ; Morimoto, Ryo ; Kennedy, Michael R. ; Layman, Lawrence C. ; Honma, Seijiro ; Sasano, Hironobu ; Rainey, William E. / Liquid chromatography-tandem mass spectrometry analysis of human adrenal vein 19-carbon steroids before and after ACTH stimulation. In: Journal of Clinical Endocrinology and Metabolism. 2013 ; Vol. 98, No. 3. pp. 1182-1188.
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abstract = "Context: Abroad analysis of adrenal gland-derived 19-carbon (C 19) steroids has not been reported. This is the first study that uses liquid chromatography-tandem mass spectrometry to quantify 9 C19 steroids (androgens and their precursors), estrone, and estradiol in the adrenal vein (AV) of women, before and after ACTH stimulation. Objective: The objective of this study was to define the adrenal androgen metabolome in women before and after ACTH infusion. Design: This was a retrospective study. Patients: Seven women, aged 50.4±5.4 years, with suspected diagnosis of an adrenal aldosterone-producing adenoma were included in the study. Methods: AV and iliac serum samples were collected before and after administration of ACTH (15 minutes). AV samples were analyzed using for concentrations of 9 unconjugated C19 steroids, estrone, and estradiol. Dehydroepiandrosterone sulfate (DHEA-S) was quantified by radioimmunoassay. Results: AV levels of DHEA-S were the highest among the steroids measured. The most abundant unconjugated C 19 steroids in AV were 11β-hydroxyandrostenedione (11OHA), dehydroepiandrosterone (DHEA), and androstenedione (A4). ACTH significantly increased the adrenal output of 9 of the 12 steroids that were measured. ACTH increased the mean AV concentration of DHEA-S by 5-fold, DHEA by 21-fold, A4 by 7-fold, and 11OHA by 5-fold. 11β-Hydroxytestosterone and testosterone were found to be potent androgen receptor agonists when tested with an androgen-responsive cell reporter model. Conclusion: The current study indicates that the adrenal gland secretes primarily 3 weak androgens, namely DHEA, 11OHA, and A4. Active androgens, including testosterone and 11β- hydroxytestosterone, are also produced but to a lesser degree.",
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AU - Kennedy, Michael R.

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AU - Honma, Seijiro

AU - Sasano, Hironobu

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