Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit

Carl R. Urbinati, Graydon Bennett Gonsalvez, John P. Aris, Roy M. Long

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Loc1p is an exclusively nuclear dsRNA-binding protein that affects the asymmetric sorting of ASH1 mRNA to daughter cells in Saccharomyces cerevisiae. In addition to the role in cytoplasmic RNA localization, Loc1p is a constituent of pre-60S ribosomes. Cells devoid of Loc1p display a defect in the synthesis of 60S ribosomal subunits, resulting in "half-mer" polyribosomes. Previously, we reported that Loc1p is located throughout the entire nucleus; however, upon closer inspection we discovered that Loc1p is enriched in the nucleolus consistent with a role in 60S ribosome biogenesis. Given that Loc1p is an RNA-binding protein and presumably functions in the assembly of 60S ribosomal subunits, we investigated if Loc1p has a role in rRNA processing and nuclear export of 60S subunits. Analysis of pre-rRNA processing revealed that loc1Δ cells exhibit gross defects in 25S rRNA synthesis, specifically a delay in processing at sites A0, A1 and A2 in 35S pre-rRNA. Furthermore, loc1Δ cells exhibit nuclear export defects for 60S ribosomal subunits, again, consistent with a role for Loc1p in the assembly of 60S ribosomal subunits. It is attractive to hypothesize that the two phenotypes associated with loc1Δ cells, namely altered ASH1 mRNA localization and ribosome biogenesis, are not mutually exclusive, but that ribosome biogenesis directly impacts mRNA localization.

Original languageEnglish (US)
Pages (from-to)369-377
Number of pages9
JournalMolecular Genetics and Genomics
Volume276
Issue number4
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

Fingerprint

Eukaryotic Large Ribosome Subunits
Cell Nucleus Active Transport
Ribosomes
RNA Precursors
RNA Transport
Messenger RNA
RNA-Binding Proteins
Polyribosomes
varespladib methyl
Saccharomyces cerevisiae
Carrier Proteins
RNA
Phenotype

Keywords

  • Ribosome biogenesis
  • RNA-binding protein
  • rRNA processing

ASJC Scopus subject areas

  • Genetics

Cite this

Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit. / Urbinati, Carl R.; Gonsalvez, Graydon Bennett; Aris, John P.; Long, Roy M.

In: Molecular Genetics and Genomics, Vol. 276, No. 4, 01.10.2006, p. 369-377.

Research output: Contribution to journalArticle

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