Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in golgi and plasma membrane defines the existence of two pools of active enzyme

David J Fulton, Jason Fontana, Grzegorz Sowa, Jean Philippe Gratton, Michelle Lin, Kai Xun Li, Belinda Michell, Bruce E. Kemp, David Rodman, William C. Sessa

Research output: Contribution to journalArticle

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Abstract

The subcellular localization of endothelial nitric-oxide synthase (eNOS) is critical for optimal coupling of extracellular stimulation to nitric oxide production. Because eNOS is activated by Akt-dependent phosphorylation to produce nitric oxide (NO), we determined the subcellular distribution of eNOS phosphorylated on serine 1179 using a variety of methodologies. Based on sucrose gradient fractionation, phosphorylated-eNOS (P-eNOS) was found in both caveolin-1-enriched membranes and intracellular domains. Co-transfection of eNOS with Akt and stimulation of endothelial cells with vascular endothelial growth factor (VEGF) increased the ratio of P-eNOS to total eNOS but did not change the relative intracellular distribution between these domains. The proper localization of eNOS to intracellular membranes was required for agonist-dependent phosphorylation on serine 1179, since VEGF did not increase eNOS phosphorylation in cells transfected with a non-acylated, mistargeted form of eNOS. Confocal imaging of P-eNOS and total eNOS pools demonstrated co-localization in the Golgi region and plasmalemma of transfected cells and native endothelial cells. Finally, VEGF stimulated a large increase in NO localized in both the perinuclear region and the plasma membrane of endothelial cells. Thus, activated, phosphorylated eNOS resides in two cellular compartments and both pools are VEGF-regulated to produce NO.

Original languageEnglish (US)
Pages (from-to)4277-4284
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number6
DOIs
StatePublished - Feb 8 2002
Externally publishedYes

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Nitric Oxide Synthase Type III
Cell membranes
Serine
Nitric Oxide
Cell Membrane
Enzymes
Vascular Endothelial Growth Factor A
Phosphorylation
Endothelial cells
Intracellular Membranes
Endothelial Cells
Membranes
Caveolin 1
Fractionation
Transfection
Sucrose
Imaging techniques

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in golgi and plasma membrane defines the existence of two pools of active enzyme. / Fulton, David J; Fontana, Jason; Sowa, Grzegorz; Gratton, Jean Philippe; Lin, Michelle; Li, Kai Xun; Michell, Belinda; Kemp, Bruce E.; Rodman, David; Sessa, William C.

In: Journal of Biological Chemistry, Vol. 277, No. 6, 08.02.2002, p. 4277-4284.

Research output: Contribution to journalArticle

Fulton, David J ; Fontana, Jason ; Sowa, Grzegorz ; Gratton, Jean Philippe ; Lin, Michelle ; Li, Kai Xun ; Michell, Belinda ; Kemp, Bruce E. ; Rodman, David ; Sessa, William C. / Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in golgi and plasma membrane defines the existence of two pools of active enzyme. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 6. pp. 4277-4284.
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