Localization of fibronectin in megakaryocytes of fetal liver

Dale E. Bockman, Adarsh K Gulati

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Antibodies specific for fibronectin were utilized to determine the sites of localization in the liver during development. The livers of fetal rats from each of gestation days 11‐19, and from days 1 and 8 postpartum, were studied by fluorescence microscopy. Fibronectin was localized predominatly in megakaryocytes and megakaryocyte precursors, and to a lesser extent in the extracellular matrix surrounding blood vessels and between hepatocytes and sinusoids. The cytoplasm of megakaryocytes and their precursors displayed bright fluorescence but their nuclei were negative for fibronectin. Hepatocytes had negative or faint fluorescence. Megakaryocytes were present in the liver from day 12, and were numerous from day 13 through most of the rest of gestation. The relative numbers of megakaryocytes decreased in later gestation; at 8 days postpartum only a few were observed per section. Hepatic megakaryocytes appeared before megakaryocytes were established in spleen and bone marrow. The early and persistent high levels of fibronectin in hepatic megakaryocytes, in the absence of comparable localization within hepatocytes, leads us to the hypothesis that megakaryocytes are important in establishing circulating fibronectin levels in the fetus. Similarly, bone marrow megakaryocytes may contribute to circulating fibronectin in the adult.

Original languageEnglish (US)
Pages (from-to)90-94
Number of pages5
JournalThe Anatomical Record
Volume223
Issue number1
DOIs
StatePublished - Jan 1 1989

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Megakaryocytes
Fibronectins
Liver
Hepatocytes
Pregnancy
Postpartum Period
Fluorescence
Bone Marrow
Fluorescence Microscopy
Extracellular Matrix
Blood Vessels
Cytoplasm
Fetus
Spleen

ASJC Scopus subject areas

  • Anatomy

Cite this

Localization of fibronectin in megakaryocytes of fetal liver. / Bockman, Dale E.; Gulati, Adarsh K.

In: The Anatomical Record, Vol. 223, No. 1, 01.01.1989, p. 90-94.

Research output: Contribution to journalArticle

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