Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia

Toshio Ariga; Susumu Kusunoki; Koh Asano; Mieko Oshima; Masahide Asano; Toru Mannen; Robert K. Yu

doi:10.1016/0006-8993(90)90060-O

Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia

Toshio Ariga, Susumu Kusunoki, Koh Asano, Mieko Oshima, Masahide Asano, Toru Mannen, Robert K. Yu

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Sulfated glucuronyl glycolipids (SGGLs) in human dorsal root ganglion (DRG)and sympathetic ganglion (SG) were analyzed biochemically and immunohistochemically. SGGLs were enriched in human DRG (1.02 ± 0.23 μg/mg protein), whereas much lower concentrations of these glycolipids (0.043 ± 0.23 μg/mg protein) were detected in SG. Myelin within DRG and SG was immunostained by anti-SGGL antiserum, although only a few myelinated fibers were seen in SG. Nerve cell bodies or unmyelinated fibers were not immunostained. Subcellular fractionation study of human DRG demonstrated that these glycolipids were not only enriched in myelin but also in the axolemma-enriched fraction. These data are consistent with the view that SGGLs may be expressed on myelinated fibers in myelin and axolemma, suggesting that these compounds may play an important role in regulating myelinogenesis.

Original language	English (US)
Pages (from-to)	57-64
Number of pages	8
Journal	Brain Research
Volume	519
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(90)90060-O
State	Published - Jun 11 1990
Externally published	Yes

Keywords

Anti-myelin-associated glycoprotein antibody
Demyetinating neuropathy
Dorsal root ganglion
Human
Sulfated glucuronyl paragloboside
Sympathetic ganglion

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(90)90060-O

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@article{d02ae175cf62490daa2ea72eee6cfe45,

title = "Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia",

abstract = "Sulfated glucuronyl glycolipids (SGGLs) in human dorsal root ganglion (DRG)and sympathetic ganglion (SG) were analyzed biochemically and immunohistochemically. SGGLs were enriched in human DRG (1.02 ± 0.23 μg/mg protein), whereas much lower concentrations of these glycolipids (0.043 ± 0.23 μg/mg protein) were detected in SG. Myelin within DRG and SG was immunostained by anti-SGGL antiserum, although only a few myelinated fibers were seen in SG. Nerve cell bodies or unmyelinated fibers were not immunostained. Subcellular fractionation study of human DRG demonstrated that these glycolipids were not only enriched in myelin but also in the axolemma-enriched fraction. These data are consistent with the view that SGGLs may be expressed on myelinated fibers in myelin and axolemma, suggesting that these compounds may play an important role in regulating myelinogenesis.",

keywords = "Anti-myelin-associated glycoprotein antibody, Demyetinating neuropathy, Dorsal root ganglion, Human, Sulfated glucuronyl paragloboside, Sympathetic ganglion",

author = "Toshio Ariga and Susumu Kusunoki and Koh Asano and Mieko Oshima and Masahide Asano and Toru Mannen and Yu, {Robert K.}",

note = "Funding Information: AcknowledgemeTnht.e authorsw isht o thankD r. S. Ando, the Tokyo MetropolitanIn stituteo f Gerontologyf,o r supplyingth e sampleo f sialylp araglobosid(LeM 1). This workw ass upporteidn partb y a grant-in-aifdo r scientificre searchfr omt he Ministryo f EducationS,c iencaen dC ultureo fJ apan( toT .A. andS .K.), a grant from the YamanouchFio undationfo r Researcho n Metabolic Disorder(sS .K.);a grant( 61-A)f romN ationaCl enteor f Neurology andP sychiatr(yN CNP)of the Ministryo f Healtha ndW elfareo f Japan (T.M.); and USPHS GrantsN S-11853a nd NS-26994(t o R.K.Y.).",

year = "1990",

month = jun,

day = "11",

doi = "10.1016/0006-8993(90)90060-O",

language = "English (US)",

volume = "519",

pages = "57--64",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia

AU - Ariga, Toshio

AU - Kusunoki, Susumu

AU - Asano, Koh

AU - Oshima, Mieko

AU - Asano, Masahide

AU - Mannen, Toru

AU - Yu, Robert K.

N1 - Funding Information: AcknowledgemeTnht.e authorsw isht o thankD r. S. Ando, the Tokyo MetropolitanIn stituteo f Gerontologyf,o r supplyingth e sampleo f sialylp araglobosid(LeM 1). This workw ass upporteidn partb y a grant-in-aifdo r scientificre searchfr omt he Ministryo f EducationS,c iencaen dC ultureo fJ apan( toT .A. andS .K.), a grant from the YamanouchFio undationfo r Researcho n Metabolic Disorder(sS .K.);a grant( 61-A)f romN ationaCl enteor f Neurology andP sychiatr(yN CNP)of the Ministryo f Healtha ndW elfareo f Japan (T.M.); and USPHS GrantsN S-11853a nd NS-26994(t o R.K.Y.).

PY - 1990/6/11

Y1 - 1990/6/11

N2 - Sulfated glucuronyl glycolipids (SGGLs) in human dorsal root ganglion (DRG)and sympathetic ganglion (SG) were analyzed biochemically and immunohistochemically. SGGLs were enriched in human DRG (1.02 ± 0.23 μg/mg protein), whereas much lower concentrations of these glycolipids (0.043 ± 0.23 μg/mg protein) were detected in SG. Myelin within DRG and SG was immunostained by anti-SGGL antiserum, although only a few myelinated fibers were seen in SG. Nerve cell bodies or unmyelinated fibers were not immunostained. Subcellular fractionation study of human DRG demonstrated that these glycolipids were not only enriched in myelin but also in the axolemma-enriched fraction. These data are consistent with the view that SGGLs may be expressed on myelinated fibers in myelin and axolemma, suggesting that these compounds may play an important role in regulating myelinogenesis.

AB - Sulfated glucuronyl glycolipids (SGGLs) in human dorsal root ganglion (DRG)and sympathetic ganglion (SG) were analyzed biochemically and immunohistochemically. SGGLs were enriched in human DRG (1.02 ± 0.23 μg/mg protein), whereas much lower concentrations of these glycolipids (0.043 ± 0.23 μg/mg protein) were detected in SG. Myelin within DRG and SG was immunostained by anti-SGGL antiserum, although only a few myelinated fibers were seen in SG. Nerve cell bodies or unmyelinated fibers were not immunostained. Subcellular fractionation study of human DRG demonstrated that these glycolipids were not only enriched in myelin but also in the axolemma-enriched fraction. These data are consistent with the view that SGGLs may be expressed on myelinated fibers in myelin and axolemma, suggesting that these compounds may play an important role in regulating myelinogenesis.

KW - Anti-myelin-associated glycoprotein antibody

KW - Demyetinating neuropathy

KW - Dorsal root ganglion

KW - Human

KW - Sulfated glucuronyl paragloboside

KW - Sympathetic ganglion

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UR - http://www.scopus.com/inward/citedby.url?scp=0025375529&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(90)90060-O

DO - 10.1016/0006-8993(90)90060-O

M3 - Article

C2 - 2204469

AN - SCOPUS:0025375529

SN - 0006-8993

VL - 519

SP - 57

EP - 64

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia

Abstract

Keywords

ASJC Scopus subject areas

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