Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia

Toshio Ariga, Susumu Kusunoki, Koh Asano, Mieko Oshima, Masahide Asano, Toru Mannen, Robert K Yu

Research output: Contribution to journalArticle

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Abstract

Sulfated glucuronyl glycolipids (SGGLs) in human dorsal root ganglion (DRG)and sympathetic ganglion (SG) were analyzed biochemically and immunohistochemically. SGGLs were enriched in human DRG (1.02 ± 0.23 μg/mg protein), whereas much lower concentrations of these glycolipids (0.043 ± 0.23 μg/mg protein) were detected in SG. Myelin within DRG and SG was immunostained by anti-SGGL antiserum, although only a few myelinated fibers were seen in SG. Nerve cell bodies or unmyelinated fibers were not immunostained. Subcellular fractionation study of human DRG demonstrated that these glycolipids were not only enriched in myelin but also in the axolemma-enriched fraction. These data are consistent with the view that SGGLs may be expressed on myelinated fibers in myelin and axolemma, suggesting that these compounds may play an important role in regulating myelinogenesis.

Original languageEnglish (US)
Pages (from-to)57-64
Number of pages8
JournalBrain Research
Volume519
Issue number1-2
DOIs
StatePublished - Jun 11 1990
Externally publishedYes

Fingerprint

Sympathetic Ganglia
Spinal Ganglia
Myelin Sheath
Glycolipids
Immune Sera
Proteins
Neurons
sulfoglycolipids

Keywords

  • Anti-myelin-associated glycoprotein antibody
  • Demyetinating neuropathy
  • Dorsal root ganglion
  • Human
  • Sulfated glucuronyl paragloboside
  • Sympathetic ganglion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia. / Ariga, Toshio; Kusunoki, Susumu; Asano, Koh; Oshima, Mieko; Asano, Masahide; Mannen, Toru; Yu, Robert K.

In: Brain Research, Vol. 519, No. 1-2, 11.06.1990, p. 57-64.

Research output: Contribution to journalArticle

Ariga, T, Kusunoki, S, Asano, K, Oshima, M, Asano, M, Mannen, T & Yu, RK 1990, 'Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia', Brain Research, vol. 519, no. 1-2, pp. 57-64. https://doi.org/10.1016/0006-8993(90)90060-O
Ariga, Toshio ; Kusunoki, Susumu ; Asano, Koh ; Oshima, Mieko ; Asano, Masahide ; Mannen, Toru ; Yu, Robert K. / Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia. In: Brain Research. 1990 ; Vol. 519, No. 1-2. pp. 57-64.
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AB - Sulfated glucuronyl glycolipids (SGGLs) in human dorsal root ganglion (DRG)and sympathetic ganglion (SG) were analyzed biochemically and immunohistochemically. SGGLs were enriched in human DRG (1.02 ± 0.23 μg/mg protein), whereas much lower concentrations of these glycolipids (0.043 ± 0.23 μg/mg protein) were detected in SG. Myelin within DRG and SG was immunostained by anti-SGGL antiserum, although only a few myelinated fibers were seen in SG. Nerve cell bodies or unmyelinated fibers were not immunostained. Subcellular fractionation study of human DRG demonstrated that these glycolipids were not only enriched in myelin but also in the axolemma-enriched fraction. These data are consistent with the view that SGGLs may be expressed on myelinated fibers in myelin and axolemma, suggesting that these compounds may play an important role in regulating myelinogenesis.

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