Localization of the vasopressin V(1a) and V2 receptors within the renal cortical and medullary circulation

Frank Park, David L. Mattson, Meredith M. Skelton, Allen W. Cowley

Research output: Contribution to journalArticle

Abstract

Arginine vasopressin (AVP) is a potent vasoconstrictor that preferentially reduces renal medullary blood flow through the stimulation of the vasopressin V(1a) receptor (V(1a)R). Studies have also shown that the vasopressin V2 receptor (V2R) may modulate AVP-mediated vasoconstriction. At present, the distribution of the V(1a)R and V2R within the renal cortical and medullary microcirculation has not been determined. This study was designed to localize the transcriptional and translational sites of the V(1a)R and V2R in microdissected intrarenal vascular segments from both the cortex and medulla, specifically the interlobar, arcuate, and interlobular arteries; afferent and efferent arterioles; glomeruli; and single outer medullary vase recta capillaries using reverse transcription-polymerase chain reaction and Western blot analyses. The results indicated that V(1a)R mRNA and proteins were present in the isolated cortical or medullary vasculature, but the V2R mRNA and proteins were not found. This study suggests that the vasoconstrictor action of AVP within the renal medulla is mediated through the V(1a)R and that the modulatory V2R-mediated vasodilation is probably through the release of paracrine hormones found within the renal interstitial or tubular cells.

Original languageEnglish (US)
Pages (from-to)R243-R251
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume273
Issue number1 42-1
StatePublished - Jul 1 1997
Externally publishedYes

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Vasopressin Receptors
Vasopressins
Arginine Vasopressin
Kidney
Vasoconstrictor Agents
Messenger RNA
Renal Circulation
Arterioles
Microcirculation
Vasoconstriction
Rectum
Vasodilation
Reverse Transcription
Blood Vessels
Proteins
Arteries
Western Blotting
Hormones
Polymerase Chain Reaction

Keywords

  • Blood vessel
  • Microdissection
  • Reverse transcription-polymerase chain reaction
  • Vasa recta capillaries
  • Western blot

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Localization of the vasopressin V(1a) and V2 receptors within the renal cortical and medullary circulation. / Park, Frank; Mattson, David L.; Skelton, Meredith M.; Cowley, Allen W.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 273, No. 1 42-1, 01.07.1997, p. R243-R251.

Research output: Contribution to journalArticle

Park, Frank ; Mattson, David L. ; Skelton, Meredith M. ; Cowley, Allen W. / Localization of the vasopressin V(1a) and V2 receptors within the renal cortical and medullary circulation. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 1997 ; Vol. 273, No. 1 42-1. pp. R243-R251.
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