Long non-coding RNAs transcribed by ERV-9 LTR retrotransposon act in cis to modulate long-range LTR enhancer function

Tianxiang Hu, Wenhu Pi, Xingguo Zhu, Miao Yu, Hongseok Ha, Huidong Shi, Jeong-Hyeon Choi, Dorothy Tuan Lo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

LTR retrotransposons are repetitive DNA elements comprising 10% of the human genome. However, LTR sequences are disproportionately present in human long, non-coding RNAs (lncRNAs). Whether and how the LTR lncRNAs serve biological functions are largely unknown. Here we show that in primary human erythroblasts, lncRNAs transcribed from the LTR retrotransposons of ERV-9 human endogenous retrovirus activated transcription of key erythroid genes and modulated ex vivo erythropoiesis. To dissect the functionalmechanism of ERV-9 lncRNAs, we performed genome-wide RNA and ChIRP analyses before and after global knockdown or locus-specific deletion of ERV-9 lncRNAs in human erythroblasts carrying 4000 copies of the ERV-9 LTRs and in transgenic mouse erythroblasts carrying a single copy of the primate-specific ERV-9 LTR in the 100 kb human ß-globin gene locus. We found that ERV-9 lncRNAs acted in cis to stabilize assembly of the ERV- 9 LTR enhancer complex and facilitate long-range LTR enhancer function in activating transcription of downstream, cis-linked globin genes. Our findings suggested that LTR lncRNAs transcribed from many of the 4000 copies of ERV-9 LTR retrotransposons acted by a similar cis mechanism tomodulate LTR enhancer function in activating transcription of downstream genes critical to cellular processes including erythropoiesis.

Original languageEnglish (US)
Pages (from-to)4479-4492
Number of pages14
JournalNucleic Acids Research
Volume45
Issue number8
DOIs
StatePublished - Jan 1 2017

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Long Noncoding RNA
Endogenous Retroviruses
Retroelements
Erythroblasts
Globins
Erythropoiesis
Genes
Human Genome
Primates
Transgenic Mice
Genome
RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Long non-coding RNAs transcribed by ERV-9 LTR retrotransposon act in cis to modulate long-range LTR enhancer function. / Hu, Tianxiang; Pi, Wenhu; Zhu, Xingguo; Yu, Miao; Ha, Hongseok; Shi, Huidong; Choi, Jeong-Hyeon; Tuan Lo, Dorothy.

In: Nucleic Acids Research, Vol. 45, No. 8, 01.01.2017, p. 4479-4492.

Research output: Contribution to journalArticle

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AU - Yu, Miao

AU - Ha, Hongseok

AU - Shi, Huidong

AU - Choi, Jeong-Hyeon

AU - Tuan Lo, Dorothy

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