Long-term measurement of arterial blood pressure in conscious mice

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Abstract

This study describes a technique for the direct daily measurement of arterial blood pressure, sampling of arterial blood, and continuous intravenous infusion in free-moving, conscious, Swiss-Webster mice. Catheters were chronically implanted in the femoral artery and vein, tunneled subcutaneously, exteriorized at the back of the neck in a lightweight tethering spring, and attached to a swivel device at the top of the cage. Time-control experiments (n = 8) demonstrated stable values of mean arterial pressure (MAP, 116 ± 1 mmHg) and heart rate (HR, 627 ± 21 beats/min) for up to 35 days after catheter implantation. It was further observed that restraining mice (n = 7) increased MAP by 10 ± 3 mmHg and HR by 78 ± 8 beats/min from the values observed under free-moving conditions. To demonstrate the chronic use of the venous catheter, intravenous infusion of N(G)-nitro-L-arginine methyl ester (L-NAME, 8.6 mg · kg-1 · day-1, n = 6) for 5 days significantly increased MAP from 117 ± 4 to 131 ± 4 mmHg without altering HR. In a final group of mice (n = 5), oral L-arginine (2% in drinking water) increased plasma arginine concentration from 90 ± 7 to 131 ± 17 μM and prevented L-NAME hypertension. These experiments illustrate the feasibility of long-term intravenous infusion, direct arterial blood pressure measurements, and arterial blood sampling in conscious mice.

Original languageEnglish (US)
Pages (from-to)R564-R570
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume274
Issue number2 43-2
StatePublished - Feb 1 1998
Externally publishedYes

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Keywords

  • Arginine
  • Hypertension
  • Nitric oxide

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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