Long-term mechanical properties of EDTA-demineralized dentin matrix

Ricardo M. Carvalho, Franklin Chi Meng Tay, Hidehiko Sano, Masahiro Yoshiyama, David Henry Pashley

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Purpose: To determine the long-term ultimate tensile strength (UTS) and modulus of elasticity (E) of EDTA-demineralized human dentin after storage in PBS (phosphate buffered saline) for up to 48 months. Materials and Methods: Dentin beams measuring 0.7 × 0.7 × 8.0 mm were prepared from the crowns of extracted human third molars. The ends of the beams were covered with resin composite and demineralized for 6 days in 0.5 mol/L EDTA (pH = 7.0). Demineralized control specimens were subjected to tensile testing at 0.5 mm/min after 24 hours of immersion in PBS solution. Experimental specimens were stored in PBS at room temperature (25°C) and tested after 18 and 48 months. The maximum load at failure and the load/displacement curves were used to calculate the UTS as a function of the cross-sectional area and E, respectively. The results were analyzed by ANOVA and Student Neuman Keuis tests. TEM micrographs were obtained from the control specimens and from those that were stored and tested after 48 months. Results: No significant changes in either UTS or E were observed in the specimens after long-term storage. The 48-month values were not statistically significantly different from the values obtained at 24 hours (p > 0.05). TEM images revealed a normal, intact structure of the collagen fibrils with no signs of degradation or denaturation. Conclusion: Long-term storage of EDTA-demineralized human dentin in PBS solution did not cause any significant reduction of its mechanical properties. The structure of the collagen fibrils as observed by TEM appeared normal and did not seem affected by long-term storage.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalJournal of Adhesive Dentistry
Volume2
Issue number3
StatePublished - Dec 1 2000

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Edetic Acid
Tensile Strength
Dentin
Phosphates
Sodium Chloride
Collagen
Third Molar
Composite Resins
Elastic Modulus
Immersion
Crowns
Analysis of Variance
Students
Temperature
Demineralized Dentin Matrix

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Periodontics

Cite this

Carvalho, R. M., Tay, F. C. M., Sano, H., Yoshiyama, M., & Pashley, D. H. (2000). Long-term mechanical properties of EDTA-demineralized dentin matrix. Journal of Adhesive Dentistry, 2(3), 193-199.

Long-term mechanical properties of EDTA-demineralized dentin matrix. / Carvalho, Ricardo M.; Tay, Franklin Chi Meng; Sano, Hidehiko; Yoshiyama, Masahiro; Pashley, David Henry.

In: Journal of Adhesive Dentistry, Vol. 2, No. 3, 01.12.2000, p. 193-199.

Research output: Contribution to journalArticle

Carvalho, RM, Tay, FCM, Sano, H, Yoshiyama, M & Pashley, DH 2000, 'Long-term mechanical properties of EDTA-demineralized dentin matrix', Journal of Adhesive Dentistry, vol. 2, no. 3, pp. 193-199.
Carvalho RM, Tay FCM, Sano H, Yoshiyama M, Pashley DH. Long-term mechanical properties of EDTA-demineralized dentin matrix. Journal of Adhesive Dentistry. 2000 Dec 1;2(3):193-199.
Carvalho, Ricardo M. ; Tay, Franklin Chi Meng ; Sano, Hidehiko ; Yoshiyama, Masahiro ; Pashley, David Henry. / Long-term mechanical properties of EDTA-demineralized dentin matrix. In: Journal of Adhesive Dentistry. 2000 ; Vol. 2, No. 3. pp. 193-199.
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