Low access resistance perforated patch recordings using amphotericin B

James Rae, Kim Cooper, Peter Gates, Mitchell Aaron Watsky

Research output: Contribution to journalArticle

786 Scopus citations

Abstract

We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were achieved and with blunt electrode tips, access resistances of 3-4 MΩ were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.

Original languageEnglish (US)
Pages (from-to)15-26
Number of pages12
JournalJournal of Neuroscience Methods
Volume37
Issue number1
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Keywords

  • Amphotericin
  • Nystatin
  • Patch clamp
  • Perforated patch
  • Resting voltage
  • Single channels
  • Whole cell currents

ASJC Scopus subject areas

  • Neuroscience(all)

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