Lower receptor avidity required for thymic clonal deletion than for effector T-cell function

Hanspeter Pircher, Urs Hoffmann Rohrer, Dimitrios Moskofidis, Rolf M. Zinkernagel, Hans Hengartner

Research output: Contribution to journalArticle

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Abstract

CLONAL deletion in the thymus plays a major part in T-cell tolerance to self antigens1-3. But the mechanism of negative selection, its fine specificity and the threshold of affinity and avidity remains unknown. We have now examined these aspects of negative selection with mice expressing a transgenic T-cell receptor with specificity for lymphocytic choriomeningitis virus (LCMV) glycoprotein in association with the class I H-2Db molecule. These mice were rendered tolerant to LCMV by neonatal infection with mutant LCMVs hearing point mutations in the T-cell epitope recognized by the transgenic T-cell receptor (ref. 4). Variant LCMVs were also tested for their ability to elicit antiviral responses in transgenic mice in vivo and in vitro. Comparison in vivo revealed that a low-avidity receptor interaction, which was unable to induce effector T cells in the periphery, was still sufficient for clonal deletion in the thymus.

Original languageEnglish (US)
Pages (from-to)482-485
Number of pages4
JournalNature
Volume351
Issue number6326
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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Clonal Deletion
Lymphocytic choriomeningitis virus
T-Lymphocytes
Thymus Gland
T-Cell Antigen Receptor Specificity
Aptitude
T-Lymphocyte Epitopes
Virus Diseases
T-Cell Antigen Receptor
Point Mutation
Transgenic Mice
Hearing
Antiviral Agents
Glycoproteins

ASJC Scopus subject areas

  • General

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Lower receptor avidity required for thymic clonal deletion than for effector T-cell function. / Pircher, Hanspeter; Rohrer, Urs Hoffmann; Moskofidis, Dimitrios; Zinkernagel, Rolf M.; Hengartner, Hans.

In: Nature, Vol. 351, No. 6326, 01.01.1991, p. 482-485.

Research output: Contribution to journalArticle

Pircher, H, Rohrer, UH, Moskofidis, D, Zinkernagel, RM & Hengartner, H 1991, 'Lower receptor avidity required for thymic clonal deletion than for effector T-cell function', Nature, vol. 351, no. 6326, pp. 482-485. https://doi.org/10.1038/351482a0
Pircher, Hanspeter ; Rohrer, Urs Hoffmann ; Moskofidis, Dimitrios ; Zinkernagel, Rolf M. ; Hengartner, Hans. / Lower receptor avidity required for thymic clonal deletion than for effector T-cell function. In: Nature. 1991 ; Vol. 351, No. 6326. pp. 482-485.
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