LRP4 is critical for neuromuscular junction maintenance

Arnab Barik, Yisheng Lu, Anupama Sathyamurthy, Andrew Bowman, Chengyong Shen, Lei Li, Wen Cheng Xiong, Lin Mei

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The neuromuscular junction (NMJ) is a synapse between motor neurons and skeletal muscle fibers, and is critical for control of muscle contraction. Its formation requires neuronal agrin that acts by binding to LRP4 to stimulate MuSK. Mutations have been identified in agrin, MuSK, and LRP4 in patients with congenital myasthenic syndrome, and patients with myasthenia gravis develop antibodies against agrin, LRP4, and MuSK. However, it remains unclear whether the agrin signaling pathway is critical for NMJ maintenance because null mutation of any of the three genes is perinatal lethal. In this study, we generated imKO mice, a mutant strain whose LRP4 gene can be deleted in muscles by doxycycline (Dox) treatment. Ablation of the LRP4 gene in adult muscle enabled studies of its role in NMJ maintenance. We demonstrate that Dox treatment of P30 mice reduced muscle strength and compound muscle action potentials. AChR clusters became fragmented with diminished junctional folds and synaptic vesicles. The amplitude and frequency of miniature endplate potentials were reduced, indicating impaired neuromuscular transmission and providing cellular mechanisms of adult LRP4 deficiency. We showed that LRP4 ablation led to the loss of synaptic agrin and the 90 kDa fragments, which occurred ahead of other prejunctional and postjunctional components, suggesting that LRP4 may regulate the stability of synaptic agrin. These observations demonstrate that LRP4 is essential for maintaining the structural and functional integrity of the NMJ and that loss of muscle LRP4 in adulthood alone is sufficient to cause myasthenic symptoms.

Original languageEnglish (US)
Pages (from-to)13892-13905
Number of pages14
JournalJournal of Neuroscience
Volume34
Issue number42
DOIs
StatePublished - Oct 15 2014

Fingerprint

Agrin
Neuromuscular Junction
Maintenance
Muscles
Doxycycline
Congenital Myasthenic Syndromes
Mutant Strains Mice
Genes
Mutation
Critical Pathways
Synaptic Vesicles
Myasthenia Gravis
Skeletal Muscle Fibers
Muscle Weakness
Muscle Strength
Motor Neurons
Muscle Contraction
Synapses
Action Potentials
Antibodies

Keywords

  • AChRs
  • Agrin
  • Congenital myasthenic syndrome
  • LRP4
  • NMJ
  • Synaptic basal lamina

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Barik, A., Lu, Y., Sathyamurthy, A., Bowman, A., Shen, C., Li, L., ... Mei, L. (2014). LRP4 is critical for neuromuscular junction maintenance. Journal of Neuroscience, 34(42), 13892-13905. https://doi.org/10.1523/JNEUROSCI.1733-14.2014

LRP4 is critical for neuromuscular junction maintenance. / Barik, Arnab; Lu, Yisheng; Sathyamurthy, Anupama; Bowman, Andrew; Shen, Chengyong; Li, Lei; Xiong, Wen Cheng; Mei, Lin.

In: Journal of Neuroscience, Vol. 34, No. 42, 15.10.2014, p. 13892-13905.

Research output: Contribution to journalArticle

Barik, A, Lu, Y, Sathyamurthy, A, Bowman, A, Shen, C, Li, L, Xiong, WC & Mei, L 2014, 'LRP4 is critical for neuromuscular junction maintenance', Journal of Neuroscience, vol. 34, no. 42, pp. 13892-13905. https://doi.org/10.1523/JNEUROSCI.1733-14.2014
Barik A, Lu Y, Sathyamurthy A, Bowman A, Shen C, Li L et al. LRP4 is critical for neuromuscular junction maintenance. Journal of Neuroscience. 2014 Oct 15;34(42):13892-13905. https://doi.org/10.1523/JNEUROSCI.1733-14.2014
Barik, Arnab ; Lu, Yisheng ; Sathyamurthy, Anupama ; Bowman, Andrew ; Shen, Chengyong ; Li, Lei ; Xiong, Wen Cheng ; Mei, Lin. / LRP4 is critical for neuromuscular junction maintenance. In: Journal of Neuroscience. 2014 ; Vol. 34, No. 42. pp. 13892-13905.
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