Lupus serum and normal human serum contain anti-DNA antibodies with the same idiotypic marker

Michael P. Madaio, A. Schattner, M. Shattner, R. S. Schwartz

Research output: Contribution to journalArticle

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Abstract

We have previously shown that serum from patients with active SLE contain high levels of Id-16/6 and anti-DNA antibodies. In this study we investigated whether serum Id 16/6 is related to anti-DNA antibodies. Sera from 12 patients with active SLE were absorbed individually with poly(dT) cellulose (to purify anti-DNA antibodies) and rabbit (R) anti-Id-16/6 Sepharose (to purify Id 16/6 Ig). Removal of all anti-DNA activity removed most of the Id-16/6. Conversely, removal of all Id 16/6 removed most of the anti-DNA activity. Although there was no measurable anti-DNA antibody activity in normal serum, such antibodies were isolated by absorption with poly(dT) cellulose. The eluted immunoglobulins also had Id 16/6 activity. Similarly, Id 16/6 with anti-DNA activity were isolated from normal serum by absorption with R anti-Id 16/6 Sepharose. We conclude that a large fraction of anti-DNA antibodies in SLE serum are Id-16/6+, and that most Id 16/6 immunoglobulins in lupus serum have anti-DNA activity. Our observations suggest that lupus anti-DNA antibodies result from an overproduction of autoantibodies that are present in normal people.

Original languageEnglish (US)
Pages (from-to)2535-2540
Number of pages6
JournalJournal of Immunology
Volume137
Issue number8
StatePublished - Dec 1 1986

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Antinuclear Antibodies
Serum
Poly T
DNA
Cellulose
Sepharose
Immunoglobulins
Autoantibodies
Rabbits
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Madaio, M. P., Schattner, A., Shattner, M., & Schwartz, R. S. (1986). Lupus serum and normal human serum contain anti-DNA antibodies with the same idiotypic marker. Journal of Immunology, 137(8), 2535-2540.

Lupus serum and normal human serum contain anti-DNA antibodies with the same idiotypic marker. / Madaio, Michael P.; Schattner, A.; Shattner, M.; Schwartz, R. S.

In: Journal of Immunology, Vol. 137, No. 8, 01.12.1986, p. 2535-2540.

Research output: Contribution to journalArticle

Madaio, MP, Schattner, A, Shattner, M & Schwartz, RS 1986, 'Lupus serum and normal human serum contain anti-DNA antibodies with the same idiotypic marker', Journal of Immunology, vol. 137, no. 8, pp. 2535-2540.
Madaio MP, Schattner A, Shattner M, Schwartz RS. Lupus serum and normal human serum contain anti-DNA antibodies with the same idiotypic marker. Journal of Immunology. 1986 Dec 1;137(8):2535-2540.
Madaio, Michael P. ; Schattner, A. ; Shattner, M. ; Schwartz, R. S. / Lupus serum and normal human serum contain anti-DNA antibodies with the same idiotypic marker. In: Journal of Immunology. 1986 ; Vol. 137, No. 8. pp. 2535-2540.
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abstract = "We have previously shown that serum from patients with active SLE contain high levels of Id-16/6 and anti-DNA antibodies. In this study we investigated whether serum Id 16/6 is related to anti-DNA antibodies. Sera from 12 patients with active SLE were absorbed individually with poly(dT) cellulose (to purify anti-DNA antibodies) and rabbit (R) anti-Id-16/6 Sepharose (to purify Id 16/6 Ig). Removal of all anti-DNA activity removed most of the Id-16/6. Conversely, removal of all Id 16/6 removed most of the anti-DNA activity. Although there was no measurable anti-DNA antibody activity in normal serum, such antibodies were isolated by absorption with poly(dT) cellulose. The eluted immunoglobulins also had Id 16/6 activity. Similarly, Id 16/6 with anti-DNA activity were isolated from normal serum by absorption with R anti-Id 16/6 Sepharose. We conclude that a large fraction of anti-DNA antibodies in SLE serum are Id-16/6+, and that most Id 16/6 immunoglobulins in lupus serum have anti-DNA activity. Our observations suggest that lupus anti-DNA antibodies result from an overproduction of autoantibodies that are present in normal people.",
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