TY - JOUR
T1 - Lycopene, a powerful antioxidant, significantly reduces the development of the adhesion phenotype
AU - Fletcher, Nicole M.
AU - Awonuga, Awoniyi O.
AU - Saed, Mohammed G.
AU - Abu-Soud, Husam M.
AU - Diamond, Michael P.
AU - Saed, Ghassan M.
N1 - Funding Information:
This study was supported in part by NIH grant number NIH RO1 GM069941 to GMS. There are no conflicts of interest.
PY - 2014/2
Y1 - 2014/2
N2 - Postoperative adhesions are a common medical complication of gynecologic and other pelvic surgeries resulting in persistent pelvic pain, obstruction of the intestines, and even infertility. The molecular mechanisms of postoperative adhesion development remain to be elucidated. We have recently described a role for reactive oxygen species, specifically superoxide, in the development of postoperative adhesions. In this study, we sought to determine whether lycopene, a potent antioxidant, reduces markers characteristic of the adhesion phenotype. Primary fibroblast cultures from normal peritoneum and adhesion tissues were utilized to determine mRNA levels of adhesion phenotype markers type I collagen, transforming growth factor-beta 1 (TGF-β1), and vascular endothelial growth factor (VEGF) in response to lycopene (24 hours, 10 μM) treatment. There was a 2 (<50.003), 4.7 (<50.004), and 1.6 fold (<50.004) increase in mRNA levels of type I collagen, TGF-β1, and VEGF, respectively, in adhesion as compared to normal peritoneal fibroblasts. Lycopene treatment led to a 6.8 and a 12.4 fold decrease in type I collagen mRNA levels, in normal peritoneal and adhesion fibroblasts, respectively (<50.005). Lycopene treatment led to a 4.2 (<50.03) and a 4.6 (<50.05) fold decrease in VEGF mRNA levels, in normal peritoneal and adhesion fibroblasts, respectively. Lycopene treatment led to a 7.0 fold decrease in TGF-β1 mRNA levels, in adhesion fibroblasts (<50.03). A 1.9 fold decrease in TGF-β1 mRNA was observed in normal peritoneal fibroblasts in response to treatment, although it was not significant. Lycopene substantially reduced levels of adhesion phenotype markers in normal peritoneal and adhesion fibroblasts and whether it will reduce postoperative adhesions needs to be further investigated.
AB - Postoperative adhesions are a common medical complication of gynecologic and other pelvic surgeries resulting in persistent pelvic pain, obstruction of the intestines, and even infertility. The molecular mechanisms of postoperative adhesion development remain to be elucidated. We have recently described a role for reactive oxygen species, specifically superoxide, in the development of postoperative adhesions. In this study, we sought to determine whether lycopene, a potent antioxidant, reduces markers characteristic of the adhesion phenotype. Primary fibroblast cultures from normal peritoneum and adhesion tissues were utilized to determine mRNA levels of adhesion phenotype markers type I collagen, transforming growth factor-beta 1 (TGF-β1), and vascular endothelial growth factor (VEGF) in response to lycopene (24 hours, 10 μM) treatment. There was a 2 (<50.003), 4.7 (<50.004), and 1.6 fold (<50.004) increase in mRNA levels of type I collagen, TGF-β1, and VEGF, respectively, in adhesion as compared to normal peritoneal fibroblasts. Lycopene treatment led to a 6.8 and a 12.4 fold decrease in type I collagen mRNA levels, in normal peritoneal and adhesion fibroblasts, respectively (<50.005). Lycopene treatment led to a 4.2 (<50.03) and a 4.6 (<50.05) fold decrease in VEGF mRNA levels, in normal peritoneal and adhesion fibroblasts, respectively. Lycopene treatment led to a 7.0 fold decrease in TGF-β1 mRNA levels, in adhesion fibroblasts (<50.03). A 1.9 fold decrease in TGF-β1 mRNA was observed in normal peritoneal fibroblasts in response to treatment, although it was not significant. Lycopene substantially reduced levels of adhesion phenotype markers in normal peritoneal and adhesion fibroblasts and whether it will reduce postoperative adhesions needs to be further investigated.
KW - Adhesion fibroblasts
KW - Lycopene
KW - TGF-β1
KW - Type I collagen
KW - VEGF
UR - http://www.scopus.com/inward/record.url?scp=84892771679&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84892771679&partnerID=8YFLogxK
U2 - 10.3109/19396368.2013.847129
DO - 10.3109/19396368.2013.847129
M3 - Article
C2 - 24219141
AN - SCOPUS:84892771679
SN - 1939-6368
VL - 60
SP - 14
EP - 20
JO - Systems Biology in Reproductive Medicine
JF - Systems Biology in Reproductive Medicine
IS - 1
ER -