Lycopene, a powerful antioxidant, significantly reduces the development of the adhesion phenotype

Nicole M. Fletcher, Awoniyi O. Awonuga, Mohammed G. Saed, Husam M. Abu-Soud, Michael P. Diamond, Ghassan M. Saed

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Postoperative adhesions are a common medical complication of gynecologic and other pelvic surgeries resulting in persistent pelvic pain, obstruction of the intestines, and even infertility. The molecular mechanisms of postoperative adhesion development remain to be elucidated. We have recently described a role for reactive oxygen species, specifically superoxide, in the development of postoperative adhesions. In this study, we sought to determine whether lycopene, a potent antioxidant, reduces markers characteristic of the adhesion phenotype. Primary fibroblast cultures from normal peritoneum and adhesion tissues were utilized to determine mRNA levels of adhesion phenotype markers type I collagen, transforming growth factor-beta 1 (TGF-β1), and vascular endothelial growth factor (VEGF) in response to lycopene (24 hours, 10 μM) treatment. There was a 2 (<50.003), 4.7 (<50.004), and 1.6 fold (<50.004) increase in mRNA levels of type I collagen, TGF-β1, and VEGF, respectively, in adhesion as compared to normal peritoneal fibroblasts. Lycopene treatment led to a 6.8 and a 12.4 fold decrease in type I collagen mRNA levels, in normal peritoneal and adhesion fibroblasts, respectively (<50.005). Lycopene treatment led to a 4.2 (<50.03) and a 4.6 (<50.05) fold decrease in VEGF mRNA levels, in normal peritoneal and adhesion fibroblasts, respectively. Lycopene treatment led to a 7.0 fold decrease in TGF-β1 mRNA levels, in adhesion fibroblasts (<50.03). A 1.9 fold decrease in TGF-β1 mRNA was observed in normal peritoneal fibroblasts in response to treatment, although it was not significant. Lycopene substantially reduced levels of adhesion phenotype markers in normal peritoneal and adhesion fibroblasts and whether it will reduce postoperative adhesions needs to be further investigated.

Original languageEnglish (US)
Pages (from-to)14-20
Number of pages7
JournalSystems Biology in Reproductive Medicine
Volume60
Issue number1
DOIs
StatePublished - Feb 1 2014

Fingerprint

Fibroblasts
Antioxidants
Phenotype
Messenger RNA
Vascular Endothelial Growth Factor A
Transforming Growth Factor beta1
Transforming Growth Factor beta
Tissue Adhesions
Pelvic Pain
Peritoneum
Therapeutics
Collagen Type I
lycopene
Superoxides
Infertility
Intestines
Reactive Oxygen Species

Keywords

  • Adhesion fibroblasts
  • Lycopene
  • TGF-β1
  • Type I collagen
  • VEGF

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

Cite this

Lycopene, a powerful antioxidant, significantly reduces the development of the adhesion phenotype. / Fletcher, Nicole M.; Awonuga, Awoniyi O.; Saed, Mohammed G.; Abu-Soud, Husam M.; Diamond, Michael P.; Saed, Ghassan M.

In: Systems Biology in Reproductive Medicine, Vol. 60, No. 1, 01.02.2014, p. 14-20.

Research output: Contribution to journalArticle

Fletcher, Nicole M. ; Awonuga, Awoniyi O. ; Saed, Mohammed G. ; Abu-Soud, Husam M. ; Diamond, Michael P. ; Saed, Ghassan M. / Lycopene, a powerful antioxidant, significantly reduces the development of the adhesion phenotype. In: Systems Biology in Reproductive Medicine. 2014 ; Vol. 60, No. 1. pp. 14-20.
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