Lymphocyte responses to purified ragweed allergens in vitro. I. Proliferative responses in normal, newborn, agammaglobulinemic, and atopic subjects

Rebecca H. Buckley, Francie Seymour, S. Ozden Sanal, Dennis Randall Ownby, W. Gary Becker

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Abstract

To evaluate cell-mediated immune responsiveness to pollen allergens in atopic subjects, we studied the deoxyribonucleic acid (DNA) synthetic responses of their cultured lymphocytes to purified ragweed antigens E, K, and Ra-3. Since lymphocytes from some highly ragweed-sensitive subjects gave poor proliferative responses when harvested on day 6, we undertook a series of dose-response and time-course studies in atopic and control subjects. Surprisingly, vigorous DNA synthetic responses to antigen E occurred with lymphocytes from all 45 subjects, including 19 highly ragweed-sensitive atopic adults (8 immunotherapy treated, 11 untreated); 13 nonatopic controls; 4 newborns, and 9 agammaglobulinemic patients. The geometric mean of peak response counts per minute in all 45 subjects was 21,163 and in unstimulated cultures was 2,416 (p = < 0.0001). The mean day on which the maximal responses occurred was 8.7, and the mean dose eliciting the maximum responses was 59 μ/ml. Statistical comparisons of the stimulated culture data revealed no significant intergroup differences. The finding of vigorous responsiveness to these purified pollen allergens by lymphocytes from nonatopic normal, newborn, and agammaglobulinemic subjects suggests that ragweed pollen antigens are either ubiquitous and lead to cell-mediated responsiveness in all subjects with intact cell-mediated inmunity, or that they may have mitogenic properties in addition to their known antigenic properties.

Original languageEnglish (US)
Pages (from-to)70-78
Number of pages9
JournalThe Journal of Allergy and Clinical Immunology
Volume59
Issue number1
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

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Ambrosia
Allergens
Newborn Infant
Lymphocytes
Pollen
Antigens
DNA
Immunotherapy
Reaction Time
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Lymphocyte responses to purified ragweed allergens in vitro. I. Proliferative responses in normal, newborn, agammaglobulinemic, and atopic subjects. / Buckley, Rebecca H.; Seymour, Francie; Sanal, S. Ozden; Ownby, Dennis Randall; Becker, W. Gary.

In: The Journal of Allergy and Clinical Immunology, Vol. 59, No. 1, 01.01.1977, p. 70-78.

Research output: Contribution to journalArticle

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