Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function

C. L. Chen, N. Singh, F. E. Yull, D. Strayhorn, L. Van Kaer, L. D. Kerr

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

NF-κB has been implicated in the development, activation, and function of B and T lymphocytes. We have evaluated the in vivo effects of deletion of IκB-α, a major inhibitor of NF-κB, on lymphocyte development, proliferation, and function. To elucidate the long term role of IκB-α in lymphocytes, fetal liver cells of 14.5-day-old IκB-α(-/-) or wild-type embryos were transplanted into irradiated recombinase-activating gene-2-deficient mice. Within 4 wk, the IκB-α(-/-) fetal liver cells reconstitute mature B and T cell populations in the recipients comparable to those produced by wild-type fetal liver cells. However, the proliferative responses of IκB-α(-/-) B cells are enhanced, whereas those of IκB-α(-/-) T cells are reduced. The levels of IgG1, IgG2a, IgA, and IgE produced by IκB-α(-/-) B cells are elevated relative to those produced by IκB-α(+/+) or IκB-α(+/-). Moreover, the specific immune responses to OVA and the generation of germinal centers are impaired in recipients of IκB-α(-/-) fetal liver cells. These results indicate that IκB-α plays a vital role in signal transduction pathways regulating lymphocyte proliferation and also in the production of specific Ig isotypes.

Original languageEnglish (US)
Pages (from-to)5418-5427
Number of pages10
JournalJournal of Immunology
Volume165
Issue number10
StatePublished - Nov 15 2000
Externally publishedYes

Fingerprint

B-Lymphocytes
Lymphocytes
Liver
T-Lymphocytes
Recombinases
Germinal Center
Immunoglobulin A
Immunoglobulin E
Signal Transduction
Embryonic Structures
Immunoglobulin G
Population
Genes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Chen, C. L., Singh, N., Yull, F. E., Strayhorn, D., Van Kaer, L., & Kerr, L. D. (2000). Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function. Journal of Immunology, 165(10), 5418-5427.

Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function. / Chen, C. L.; Singh, N.; Yull, F. E.; Strayhorn, D.; Van Kaer, L.; Kerr, L. D.

In: Journal of Immunology, Vol. 165, No. 10, 15.11.2000, p. 5418-5427.

Research output: Contribution to journalArticle

Chen, CL, Singh, N, Yull, FE, Strayhorn, D, Van Kaer, L & Kerr, LD 2000, 'Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function', Journal of Immunology, vol. 165, no. 10, pp. 5418-5427.
Chen, C. L. ; Singh, N. ; Yull, F. E. ; Strayhorn, D. ; Van Kaer, L. ; Kerr, L. D. / Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function. In: Journal of Immunology. 2000 ; Vol. 165, No. 10. pp. 5418-5427.
@article{7420b3468444433d840802ee2e22b222,
title = "Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function",
abstract = "NF-κB has been implicated in the development, activation, and function of B and T lymphocytes. We have evaluated the in vivo effects of deletion of IκB-α, a major inhibitor of NF-κB, on lymphocyte development, proliferation, and function. To elucidate the long term role of IκB-α in lymphocytes, fetal liver cells of 14.5-day-old IκB-α(-/-) or wild-type embryos were transplanted into irradiated recombinase-activating gene-2-deficient mice. Within 4 wk, the IκB-α(-/-) fetal liver cells reconstitute mature B and T cell populations in the recipients comparable to those produced by wild-type fetal liver cells. However, the proliferative responses of IκB-α(-/-) B cells are enhanced, whereas those of IκB-α(-/-) T cells are reduced. The levels of IgG1, IgG2a, IgA, and IgE produced by IκB-α(-/-) B cells are elevated relative to those produced by IκB-α(+/+) or IκB-α(+/-). Moreover, the specific immune responses to OVA and the generation of germinal centers are impaired in recipients of IκB-α(-/-) fetal liver cells. These results indicate that IκB-α plays a vital role in signal transduction pathways regulating lymphocyte proliferation and also in the production of specific Ig isotypes.",
author = "Chen, {C. L.} and N. Singh and Yull, {F. E.} and D. Strayhorn and {Van Kaer}, L. and Kerr, {L. D.}",
year = "2000",
month = "11",
day = "15",
language = "English (US)",
volume = "165",
pages = "5418--5427",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "10",

}

TY - JOUR

T1 - Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function

AU - Chen, C. L.

AU - Singh, N.

AU - Yull, F. E.

AU - Strayhorn, D.

AU - Van Kaer, L.

AU - Kerr, L. D.

PY - 2000/11/15

Y1 - 2000/11/15

N2 - NF-κB has been implicated in the development, activation, and function of B and T lymphocytes. We have evaluated the in vivo effects of deletion of IκB-α, a major inhibitor of NF-κB, on lymphocyte development, proliferation, and function. To elucidate the long term role of IκB-α in lymphocytes, fetal liver cells of 14.5-day-old IκB-α(-/-) or wild-type embryos were transplanted into irradiated recombinase-activating gene-2-deficient mice. Within 4 wk, the IκB-α(-/-) fetal liver cells reconstitute mature B and T cell populations in the recipients comparable to those produced by wild-type fetal liver cells. However, the proliferative responses of IκB-α(-/-) B cells are enhanced, whereas those of IκB-α(-/-) T cells are reduced. The levels of IgG1, IgG2a, IgA, and IgE produced by IκB-α(-/-) B cells are elevated relative to those produced by IκB-α(+/+) or IκB-α(+/-). Moreover, the specific immune responses to OVA and the generation of germinal centers are impaired in recipients of IκB-α(-/-) fetal liver cells. These results indicate that IκB-α plays a vital role in signal transduction pathways regulating lymphocyte proliferation and also in the production of specific Ig isotypes.

AB - NF-κB has been implicated in the development, activation, and function of B and T lymphocytes. We have evaluated the in vivo effects of deletion of IκB-α, a major inhibitor of NF-κB, on lymphocyte development, proliferation, and function. To elucidate the long term role of IκB-α in lymphocytes, fetal liver cells of 14.5-day-old IκB-α(-/-) or wild-type embryos were transplanted into irradiated recombinase-activating gene-2-deficient mice. Within 4 wk, the IκB-α(-/-) fetal liver cells reconstitute mature B and T cell populations in the recipients comparable to those produced by wild-type fetal liver cells. However, the proliferative responses of IκB-α(-/-) B cells are enhanced, whereas those of IκB-α(-/-) T cells are reduced. The levels of IgG1, IgG2a, IgA, and IgE produced by IκB-α(-/-) B cells are elevated relative to those produced by IκB-α(+/+) or IκB-α(+/-). Moreover, the specific immune responses to OVA and the generation of germinal centers are impaired in recipients of IκB-α(-/-) fetal liver cells. These results indicate that IκB-α plays a vital role in signal transduction pathways regulating lymphocyte proliferation and also in the production of specific Ig isotypes.

UR - http://www.scopus.com/inward/record.url?scp=0034670001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034670001&partnerID=8YFLogxK

M3 - Article

C2 - 11067893

AN - SCOPUS:0034670001

VL - 165

SP - 5418

EP - 5427

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 10

ER -