Lysophospholipids and lysophospholipase D in rabbit aqueous humor following corneal injury

Akira Tokumura, Satoshi Taira, Masaki Kikuchi, Toshihiko Tsutsumi, Yoshibumi Shimizu, Mitchell Aaron Watsky

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We previously found that lysophosphatidic acid (LPA)-like activity eliciting Cl - currents in Xenopus oocytes is increased in rabbit aqueous humor (AH) following corneal freeze wounds. The purpose of this study was to examine whether actual levels of LPA in AH from wounded eyes are higher than those from control eyes, and to determine the sources and enzymatic pathways of AH LPA in control and wounded conditions. Lysophospholipase D (lysoPLD) activity was measured by the enzymatic determination of choline following incubation of AH samples with exogenous lysophosphatidylcholines (LPCs). The molecular species compositions of LPA and LPC in fresh and incubated AH were determined by liquid chromatography-tandem mass spectrometry. A high, but similar activity of lysoPLD in the samples from both control and freeze-wounded eyes was detected. Its enzymatic properties resemble those of plasma lysoPLD, identified as autotaxin. Levels of LPCs, predominant substrates of lysoPLD in AH, were several times higher in the AH samples from injured eyes than those from the control eyes. Our results suggest that lysoPLD is constitutively released from corneal tissues and/or ciliary body into the AH, with no injury-induced increase in release following freeze-wounding. They also suggest that wound-induced increases in LPA-like biological activity are due to linoleoyl species-rich molecular composition in AH from wounded eyes. A possible mechanism of the altered molecular composition is an increase in the AH concentrations of LPCs, linoleoyl species of which are preferentially converted to corresponding unsaturated LPA by the constitutively active lysoPLD.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalProstaglandins and Other Lipid Mediators
Volume97
Issue number3-4
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Lysophospholipids
Aqueous Humor
Lysophosphatidylcholines
Rabbits
Chemical analysis
Liquid chromatography
Choline
Bioactivity
Wounds and Injuries
Mass spectrometry
Corneal Injuries
alkylglycerophosphoethanolamine phosphodiesterase
lysophosphatidic acid
Ciliary Body
Tissue
Plasmas
Xenopus
Tandem Mass Spectrometry
Liquid Chromatography
Oocytes

Keywords

  • Aqueous humor
  • Autotoxin
  • Corneal injury
  • Lysophosphatidic acid
  • Lysophosphatidylcholine
  • Lysophospholipase D

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

Cite this

Lysophospholipids and lysophospholipase D in rabbit aqueous humor following corneal injury. / Tokumura, Akira; Taira, Satoshi; Kikuchi, Masaki; Tsutsumi, Toshihiko; Shimizu, Yoshibumi; Watsky, Mitchell Aaron.

In: Prostaglandins and Other Lipid Mediators, Vol. 97, No. 3-4, 01.03.2012, p. 83-89.

Research output: Contribution to journalArticle

Tokumura, Akira ; Taira, Satoshi ; Kikuchi, Masaki ; Tsutsumi, Toshihiko ; Shimizu, Yoshibumi ; Watsky, Mitchell Aaron. / Lysophospholipids and lysophospholipase D in rabbit aqueous humor following corneal injury. In: Prostaglandins and Other Lipid Mediators. 2012 ; Vol. 97, No. 3-4. pp. 83-89.
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