Lysosome-associated membrane protein 1 is a major SSEA-1-carrier protein in mouse neural stem cells

Hirokazu Yagi; Makoto Yanagisawa; Koichi Kato; Robert K. Yu

doi:10.1093/glycob/cwq054

Lysosome-associated membrane protein 1 is a major SSEA-1-carrier protein in mouse neural stem cells

Hirokazu Yagi, Makoto Yanagisawa, Koichi Kato, Robert K. Yu

Neuroscience and Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Stage-specific embryonic antigen-1 (SSEA-1) is a wellknown carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is a major carrier protein of SSEA-1 in NSCs, despite the common belief that SSEA-1 is mainly expressed on the cell surface and constitutes a component of the extracellular matrix. Furthermore, we found that SSEA-1 on LAMP-1 is completely ablated in differentiated cells derived from NSCs. Our finding raises the possibility that the expression of SSEA-1-positive LAMP-1 is associated with the "stemness" of NSCs.

Original language	English (US)
Article number	cwq054
Pages (from-to)	976-981
Number of pages	6
Journal	Glycobiology
Volume	20
Issue number	8
DOIs	https://doi.org/10.1093/glycob/cwq054
State	Published - Mar 31 2010

Keywords

Development
LAMP-1
N-glycosylation
Neural stem cell
SSEA-1

ASJC Scopus subject areas

Biochemistry

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10.1093/glycob/cwq054

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title = "Lysosome-associated membrane protein 1 is a major SSEA-1-carrier protein in mouse neural stem cells",

abstract = "Stage-specific embryonic antigen-1 (SSEA-1) is a wellknown carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is a major carrier protein of SSEA-1 in NSCs, despite the common belief that SSEA-1 is mainly expressed on the cell surface and constitutes a component of the extracellular matrix. Furthermore, we found that SSEA-1 on LAMP-1 is completely ablated in differentiated cells derived from NSCs. Our finding raises the possibility that the expression of SSEA-1-positive LAMP-1 is associated with the {"}stemness{"} of NSCs.",

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author = "Hirokazu Yagi and Makoto Yanagisawa and Koichi Kato and Yu, {Robert K.}",

note = "Funding Information: This study was supported by United States Public Health Service grants (NS11853, NS26994), a grant from the Childrens' Medical Research Foundation, Chicago, IL to R.K.Y. and a start-up fund awarded to M.Y. from the Medical College of Georgia. H.Y. was supported by the Japan–US Brain Research Cooperative Program.",

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AU - Yanagisawa, Makoto

AU - Kato, Koichi

AU - Yu, Robert K.

N1 - Funding Information: This study was supported by United States Public Health Service grants (NS11853, NS26994), a grant from the Childrens' Medical Research Foundation, Chicago, IL to R.K.Y. and a start-up fund awarded to M.Y. from the Medical College of Georgia. H.Y. was supported by the Japan–US Brain Research Cooperative Program.

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N2 - Stage-specific embryonic antigen-1 (SSEA-1) is a wellknown carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is a major carrier protein of SSEA-1 in NSCs, despite the common belief that SSEA-1 is mainly expressed on the cell surface and constitutes a component of the extracellular matrix. Furthermore, we found that SSEA-1 on LAMP-1 is completely ablated in differentiated cells derived from NSCs. Our finding raises the possibility that the expression of SSEA-1-positive LAMP-1 is associated with the "stemness" of NSCs.

AB - Stage-specific embryonic antigen-1 (SSEA-1) is a wellknown carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is a major carrier protein of SSEA-1 in NSCs, despite the common belief that SSEA-1 is mainly expressed on the cell surface and constitutes a component of the extracellular matrix. Furthermore, we found that SSEA-1 on LAMP-1 is completely ablated in differentiated cells derived from NSCs. Our finding raises the possibility that the expression of SSEA-1-positive LAMP-1 is associated with the "stemness" of NSCs.

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