Lysosome-associated membrane protein 1 is a major SSEA-1-carrier protein in mouse neural stem cells

Hirokazu Yagi, Makoto Yanagisawa, Koichi Kato, Robert K. Yu

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Stage-specific embryonic antigen-1 (SSEA-1) is a wellknown carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is a major carrier protein of SSEA-1 in NSCs, despite the common belief that SSEA-1 is mainly expressed on the cell surface and constitutes a component of the extracellular matrix. Furthermore, we found that SSEA-1 on LAMP-1 is completely ablated in differentiated cells derived from NSCs. Our finding raises the possibility that the expression of SSEA-1-positive LAMP-1 is associated with the "stemness" of NSCs.

Original languageEnglish (US)
Article numbercwq054
Pages (from-to)976-981
Number of pages6
JournalGlycobiology
Volume20
Issue number8
DOIs
StatePublished - Mar 31 2010

Keywords

  • Development
  • LAMP-1
  • N-glycosylation
  • Neural stem cell
  • SSEA-1

ASJC Scopus subject areas

  • Biochemistry

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