Abstract
Stage-specific embryonic antigen-1 (SSEA-1) is a wellknown carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is a major carrier protein of SSEA-1 in NSCs, despite the common belief that SSEA-1 is mainly expressed on the cell surface and constitutes a component of the extracellular matrix. Furthermore, we found that SSEA-1 on LAMP-1 is completely ablated in differentiated cells derived from NSCs. Our finding raises the possibility that the expression of SSEA-1-positive LAMP-1 is associated with the "stemness" of NSCs.
| Original language | English (US) |
|---|---|
| Article number | cwq054 |
| Pages (from-to) | 976-981 |
| Number of pages | 6 |
| Journal | Glycobiology |
| Volume | 20 |
| Issue number | 8 |
| DOIs | |
| State | Published - Mar 31 2010 |
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Keywords
- Development
- LAMP-1
- N-glycosylation
- Neural stem cell
- SSEA-1
ASJC Scopus subject areas
- Biochemistry
Cite this
Lysosome-associated membrane protein 1 is a major SSEA-1-carrier protein in mouse neural stem cells. / Yagi, Hirokazu; Yanagisawa, Makoto; Kato, Koichi; Yu, Robert K.
In: Glycobiology, Vol. 20, No. 8, cwq054, 31.03.2010, p. 976-981.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lysosome-associated membrane protein 1 is a major SSEA-1-carrier protein in mouse neural stem cells
AU - Yagi, Hirokazu
AU - Yanagisawa, Makoto
AU - Kato, Koichi
AU - Yu, Robert K
PY - 2010/3/31
Y1 - 2010/3/31
N2 - Stage-specific embryonic antigen-1 (SSEA-1) is a wellknown carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is a major carrier protein of SSEA-1 in NSCs, despite the common belief that SSEA-1 is mainly expressed on the cell surface and constitutes a component of the extracellular matrix. Furthermore, we found that SSEA-1 on LAMP-1 is completely ablated in differentiated cells derived from NSCs. Our finding raises the possibility that the expression of SSEA-1-positive LAMP-1 is associated with the "stemness" of NSCs.
AB - Stage-specific embryonic antigen-1 (SSEA-1) is a wellknown carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is a major carrier protein of SSEA-1 in NSCs, despite the common belief that SSEA-1 is mainly expressed on the cell surface and constitutes a component of the extracellular matrix. Furthermore, we found that SSEA-1 on LAMP-1 is completely ablated in differentiated cells derived from NSCs. Our finding raises the possibility that the expression of SSEA-1-positive LAMP-1 is associated with the "stemness" of NSCs.
KW - Development
KW - LAMP-1
KW - N-glycosylation
KW - Neural stem cell
KW - SSEA-1
UR - http://www.scopus.com/inward/record.url?scp=77954464336&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954464336&partnerID=8YFLogxK
U2 - 10.1093/glycob/cwq054
DO - 10.1093/glycob/cwq054
M3 - Article
C2 - 20360060
AN - SCOPUS:77954464336
VL - 20
SP - 976
EP - 981
JO - Glycobiology
JF - Glycobiology
SN - 0959-6658
IS - 8
M1 - cwq054
ER -