M6P/IGF2R is mutated in squamous cell carcinoma of the lung

Feng Ming Kong, Mitchell S. Anscher, Mary K. Washington, J. Keith Killian, Randy L. Jirtle

Research output: Contribution to journalArticlepeer-review

101 Scopus citations

Abstract

In addition to the intracellular sorting of lysosomal enzymes, the mannose 6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) plays a critical role in regulating the bioavailability of extracellular proteolytic enzymes and growth factors. It has also been shown to be mutated in a number of human cancers, and to suppress cancer cell growth. The purpose of this study was to determine if the M6P/IGF2R is mutated in lung cancer, a leading cause of cancer death worldwide. Archival pathology specimens were obtained on 22 patients with newly diagnosed, untreated squamous cell carcinoma of the lung. Two polymorphisms in the 3'-untranslated region of the M6P/IGF2R were used to screen lung tumors for loss of heterozygosity (LOH) by PCR amplification of DNA. Nineteen of 22 (86%) patients were informative (heterozygous), and 11/19 (58%) squamous cell carcinomas of the lung had LOH at the M6P/IGF2R locus. The remaining allele in 6/11 (55%) LOH patients contained mutations in either the mannose 6-phosphate or the IGF2 binding domain of the M6P/ IGF2R. Thus, the M6P/IGF2R is mutated frequently in squamous cell carcinoma of the lung, providing further support for its function as a tumor suppressor.

Original languageEnglish (US)
Pages (from-to)1572-1578
Number of pages7
JournalOncogene
Volume19
Issue number12
DOIs
StatePublished - Mar 16 2000

Keywords

  • Loss of heterozygosity
  • Lung cancer
  • M6P/IGF2R
  • Mutation
  • Tumor suppressor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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