Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex

John Clark Sutherland; John F. Duval; Kathleen Pietruszka Griffin

doi:10.1021/bi00617a003

Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex

John Clark Sutherland, John F. Duval, Kathleen Pietruszka Griffin

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14 Scopus citations

Abstract

We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.

Original language	English (US)
Pages (from-to)	5088-5091
Number of pages	4
Journal	Biochemistry
Volume	17
Issue number	24
DOIs	https://doi.org/10.1021/bi00617a003
State	Published - 1978
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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10.1021/bi00617a003

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title = "Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex",

abstract = "We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.",

author = "Sutherland, {John Clark} and Duval, {John F.} and Griffin, {Kathleen Pietruszka}",

year = "1978",

doi = "10.1021/bi00617a003",

language = "English (US)",

volume = "17",

pages = "5088--5091",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

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T1 - Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex

AU - Sutherland, John Clark

AU - Duval, John F.

AU - Griffin, Kathleen Pietruszka

PY - 1978

Y1 - 1978

N2 - We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.

AB - We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.

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JO - Biochemistry

JF - Biochemistry

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ER -

Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex

Abstract

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