Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex

John C. Sutherland, John F. Duval, Kathleen Pietruszka Griffin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.

Original languageEnglish (US)
Pages (from-to)5088-5091
Number of pages4
JournalBiochemistry
Volume17
Issue number24
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

Fingerprint

Netropsin
Circular Dichroism
DNA
Deoxyribodipyrimidine Photo-Lyase
DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Absorption spectra
Anti-Bacterial Agents
Wavelength
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex. / Sutherland, John C.; Duval, John F.; Griffin, Kathleen Pietruszka.

In: Biochemistry, Vol. 17, No. 24, 01.01.1978, p. 5088-5091.

Research output: Contribution to journalArticle

Sutherland, John C. ; Duval, John F. ; Griffin, Kathleen Pietruszka. / Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex. In: Biochemistry. 1978 ; Vol. 17, No. 24. pp. 5088-5091.
@article{a2c09320a12a402da2b8c9d6adaf4439,
title = "Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex",
abstract = "We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.",
author = "Sutherland, {John C.} and Duval, {John F.} and Griffin, {Kathleen Pietruszka}",
year = "1978",
month = "1",
day = "1",
doi = "10.1021/bi00617a003",
language = "English (US)",
volume = "17",
pages = "5088--5091",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "24",

}

TY - JOUR

T1 - Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex

AU - Sutherland, John C.

AU - Duval, John F.

AU - Griffin, Kathleen Pietruszka

PY - 1978/1/1

Y1 - 1978/1/1

N2 - We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.

AB - We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.

UR - http://www.scopus.com/inward/record.url?scp=0018278639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018278639&partnerID=8YFLogxK

U2 - 10.1021/bi00617a003

DO - 10.1021/bi00617a003

M3 - Article

VL - 17

SP - 5088

EP - 5091

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 24

ER -