Magnetic Circular Dichroism of Netropsin and Natural Circular Dichroism of the Netropsin-DNA Complex

John Clark Sutherland, John F. Duval, Kathleen Pietruszka Griffin

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We report the first measurement of the magnetic circular dichroism (MCD) of the basic polypeptide antibiotic netropsin (Nt). The MCD shows that the longest wavelength absorption band of Nt is the sum of more than one component and permits a radically new interpretation of the circular dichroism of the complex which Nt forms with DNA. We conclude that Nt has no major effect on the CD and thus the helical structure of the bases of the DNA to which it is bound. Thus the ability of Nt to inhibit the function of DNA polymerase, RNA polymerase, and the photoreactivating enzyme must be mediated by factors other than a distortion of the helical structure of the bases.

Original languageEnglish (US)
Pages (from-to)5088-5091
Number of pages4
JournalBiochemistry
Volume17
Issue number24
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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