Magnetically-labeled sensitized splenocytes to identify glioma by MRI: A preliminary study

Ali S. Arbab, Ali M. Rad, A. S.M. Iskander, Kourosh Jafari-Khouzani, Stephen L. Brown, Jamie L. Churchman, Guangliang Ding, Quan Jiang, Joseph A. Frank, Hamid Soltanian-Zadeh, Donald J. Peck

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


This study investigated the feasibility of imaging the migration and incorporation of magnetically-labeled sensitized splenocytes in an experimental 9L glioma brain tumor model. Splenocytes collected from tumor-bearing (sensitized splenocytes) or control (nonsensitized splenocytes) host rats were analyzed to determine the population of different cells, labeled with ferumoxides-protamine sulfate (FePro) and injected intravenously to recipient rats (N = 4, for each group) bearing intracranial 9L tumors. Day 3 postinjection of splenocytes multiecho T2*-weighted and three-dimensional (3D) gradient echo MRI were obtained using a 7 Tesla MR system. R 2* (1/T2*) maps were created from the T 2*-weighted images. Signal intensities (SIs) and R 2* values in the tumors and contralateral brain were determined by hand drawn regions of interest (ROIs). Brain sections were stained for the evidence of administered cells. Both 3D and T2*-weighted MRI showed low signal intensity areas in and around the tumors in rats that received labeled sensitized splenocytes. Prussian blue (PB), CD45- and CD8-positive cells were present in areas at the corresponding sites of low signal intensities seen on MRI. Rats that received labeled nonsensitized splenocytes did not show low signal intensity areas or PB positive cells in or around the implanted tumors. In conclusion, the immunogenic reaction can be exploited to delineate recurrent glioma using MRI following systemically delivered magnetically labeled sensitized splenocytes or T-cells.

Original languageEnglish (US)
Pages (from-to)519-526
Number of pages8
JournalMagnetic Resonance in Medicine
Issue number3
StatePublished - Sep 2007
Externally publishedYes


  • Glioma
  • Iron oxides
  • Magnetic labeling sensitized splenocytes
  • Magnetic resonance imaging
  • SPIO

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


Dive into the research topics of 'Magnetically-labeled sensitized splenocytes to identify glioma by MRI: A preliminary study'. Together they form a unique fingerprint.

Cite this