Manganese-gold nanoparticles as an MRI positive contrast agent in mesenchymal stem cell labeling

Simona E.Hunyadi Murph; Stephanie Jacobs; Jimei Liu; Tom C.C. Hu; Matthew Siegfired; Steven M. Serkiz; Joan Hudson

doi:10.1007/s11051-011-0658-7

Manganese-gold nanoparticles as an MRI positive contrast agent in mesenchymal stem cell labeling

Simona E.Hunyadi Murph, Stephanie Jacobs, Jimei Liu, Tom C.C. Hu, Matthew Siegfired, Steven M. Serkiz, Joan Hudson

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We report a straightforward approach to prepare multifunctional manganese-gold nanoparticles by attaching Mn(II) ions onto the surface of 20 nm citrate-capped gold nanoparticles. In vitro MRI measurements made in agarose gel phantoms exhibited high relaxivity (18.26 ± 1.04 mmol^-1 s^-1). Controlled incubation of the nanoparticles with mesenchymal stem cells (MSCs) was used to study cellular uptake of these particles and this process appeared to be controlled by the size of the nanoparticle aggregates in the extracellular solution. SEM images of live MSCs showed an increased concentration of particles near the cell membrane and a distribution of the size of particles within the cells. Survivability for MSCs in contact with Mn-Au NPs was greater than 97%over the 3-day period and up to the 1 mM Mn used in this study. The high relaxivity and low cell mortality are suggestive of an enhanced positive contrast agent for in vitro or in vivo applications.

Original language	English (US)
Article number	658
Journal	Journal of Nanoparticle Research
Volume	14
Issue number	4
DOIs	https://doi.org/10.1007/s11051-011-0658-7
State	Published - Mar 2012

Keywords

Gold
Imaging
MRI
Manganese
Multifunctional nanoparticles
Nanomedicine
Stem cell uptake
Synthesis

ASJC Scopus subject areas

Bioengineering
Atomic and Molecular Physics, and Optics
General Chemistry
Modeling and Simulation
General Materials Science
Condensed Matter Physics

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10.1007/s11051-011-0658-7

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title = "Manganese-gold nanoparticles as an MRI positive contrast agent in mesenchymal stem cell labeling",

abstract = "We report a straightforward approach to prepare multifunctional manganese-gold nanoparticles by attaching Mn(II) ions onto the surface of 20 nm citrate-capped gold nanoparticles. In vitro MRI measurements made in agarose gel phantoms exhibited high relaxivity (18.26 ± 1.04 mmol-1 s-1). Controlled incubation of the nanoparticles with mesenchymal stem cells (MSCs) was used to study cellular uptake of these particles and this process appeared to be controlled by the size of the nanoparticle aggregates in the extracellular solution. SEM images of live MSCs showed an increased concentration of particles near the cell membrane and a distribution of the size of particles within the cells. Survivability for MSCs in contact with Mn-Au NPs was greater than 97%over the 3-day period and up to the 1 mM Mn used in this study. The high relaxivity and low cell mortality are suggestive of an enhanced positive contrast agent for in vitro or in vivo applications.",

keywords = "Gold, Imaging, MRI, Manganese, Multifunctional nanoparticles, Nanomedicine, Stem cell uptake, Synthesis",

author = "Murph, {Simona E.Hunyadi} and Stephanie Jacobs and Jimei Liu and Hu, {Tom C.C.} and Matthew Siegfired and Serkiz, {Steven M.} and Joan Hudson",

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AU - Murph, Simona E.Hunyadi

AU - Jacobs, Stephanie

AU - Liu, Jimei

AU - Hu, Tom C.C.

AU - Siegfired, Matthew

AU - Serkiz, Steven M.

AU - Hudson, Joan

PY - 2012/3

Y1 - 2012/3

N2 - We report a straightforward approach to prepare multifunctional manganese-gold nanoparticles by attaching Mn(II) ions onto the surface of 20 nm citrate-capped gold nanoparticles. In vitro MRI measurements made in agarose gel phantoms exhibited high relaxivity (18.26 ± 1.04 mmol-1 s-1). Controlled incubation of the nanoparticles with mesenchymal stem cells (MSCs) was used to study cellular uptake of these particles and this process appeared to be controlled by the size of the nanoparticle aggregates in the extracellular solution. SEM images of live MSCs showed an increased concentration of particles near the cell membrane and a distribution of the size of particles within the cells. Survivability for MSCs in contact with Mn-Au NPs was greater than 97%over the 3-day period and up to the 1 mM Mn used in this study. The high relaxivity and low cell mortality are suggestive of an enhanced positive contrast agent for in vitro or in vivo applications.

AB - We report a straightforward approach to prepare multifunctional manganese-gold nanoparticles by attaching Mn(II) ions onto the surface of 20 nm citrate-capped gold nanoparticles. In vitro MRI measurements made in agarose gel phantoms exhibited high relaxivity (18.26 ± 1.04 mmol-1 s-1). Controlled incubation of the nanoparticles with mesenchymal stem cells (MSCs) was used to study cellular uptake of these particles and this process appeared to be controlled by the size of the nanoparticle aggregates in the extracellular solution. SEM images of live MSCs showed an increased concentration of particles near the cell membrane and a distribution of the size of particles within the cells. Survivability for MSCs in contact with Mn-Au NPs was greater than 97%over the 3-day period and up to the 1 mM Mn used in this study. The high relaxivity and low cell mortality are suggestive of an enhanced positive contrast agent for in vitro or in vivo applications.

KW - Gold

KW - Imaging

KW - MRI

KW - Manganese

KW - Multifunctional nanoparticles

KW - Nanomedicine

KW - Stem cell uptake

KW - Synthesis

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Manganese-gold nanoparticles as an MRI positive contrast agent in mesenchymal stem cell labeling

Abstract

Keywords

ASJC Scopus subject areas

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