Mapping the lectin-like activity of tumor necrosis factor

Rudolf Lucas, Stefan Magez, Robert De Leys, Lucie Fransen, Jean Pierre Scheerlinck, Murielle Rampelberg, Erwin Sablon, Patrick De Baetselier

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Tumor necrosis factor (TNF), but not lymphotoxin (LT), is directly trypanolytic for salivarian trypanosomes. This activity was not blocked by soluble 55-kilodalton and 75-kilodalton TNF receptors, but was potently inhibited by N,N'-diacetylchitobiose, an oligosaccharide that binds TNF. Comparative sequence analysis of TNF and LT localized the trypanocidal region, and synthetic peptides were trypanolytic. TNF molecules in which the trypanocidal region was mutated or deleted retained tumoricidal activity. Thus, trypanosome-TNF interactions occur via a TNF domain, probably with lectin-like affinity, which is functionally and spatially distinct from the mammalian TNF receptor binding sites.

Original languageEnglish (US)
Pages (from-to)814-817
Number of pages4
JournalScience
Volume263
Issue number5148
DOIs
StatePublished - Feb 11 1994
Externally publishedYes

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Lectins
Tumor Necrosis Factor-alpha
Lymphotoxin-alpha
Trypanosomiasis
Receptors, Tumor Necrosis Factor, Type II
Tumor Necrosis Factor Receptors
Oligosaccharides
Sequence Analysis
Binding Sites
Peptides

ASJC Scopus subject areas

  • General

Cite this

Lucas, R., Magez, S., De Leys, R., Fransen, L., Scheerlinck, J. P., Rampelberg, M., ... De Baetselier, P. (1994). Mapping the lectin-like activity of tumor necrosis factor. Science, 263(5148), 814-817. https://doi.org/10.1126/science.8303299

Mapping the lectin-like activity of tumor necrosis factor. / Lucas, Rudolf; Magez, Stefan; De Leys, Robert; Fransen, Lucie; Scheerlinck, Jean Pierre; Rampelberg, Murielle; Sablon, Erwin; De Baetselier, Patrick.

In: Science, Vol. 263, No. 5148, 11.02.1994, p. 814-817.

Research output: Contribution to journalArticle

Lucas, R, Magez, S, De Leys, R, Fransen, L, Scheerlinck, JP, Rampelberg, M, Sablon, E & De Baetselier, P 1994, 'Mapping the lectin-like activity of tumor necrosis factor', Science, vol. 263, no. 5148, pp. 814-817. https://doi.org/10.1126/science.8303299
Lucas R, Magez S, De Leys R, Fransen L, Scheerlinck JP, Rampelberg M et al. Mapping the lectin-like activity of tumor necrosis factor. Science. 1994 Feb 11;263(5148):814-817. https://doi.org/10.1126/science.8303299
Lucas, Rudolf ; Magez, Stefan ; De Leys, Robert ; Fransen, Lucie ; Scheerlinck, Jean Pierre ; Rampelberg, Murielle ; Sablon, Erwin ; De Baetselier, Patrick. / Mapping the lectin-like activity of tumor necrosis factor. In: Science. 1994 ; Vol. 263, No. 5148. pp. 814-817.
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