Marginal zone CD169+ macrophages coordinate apoptotic cell-driven cellular recruitment and tolerance

Buvana Ravishankar, Rahul Shinde, Haiyun Liu, Kapil Chaudhary, Jillian Bradley, Henrique P. Lemos, Chandlera Phillip, Masato Tanaka, David H. Munn, Andrew L. Mellor, Tracy L. McGaha

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Tolerance to apoptotic cells is essential to prevent inflammatory pathology. Though innate responses are critical for immune suppression, our understanding of early innate immunity driven by apoptosis is lacking. Herein we report apoptotic cells induce expression of the chemokine CCL22 in splenic metallophillic macrophages, which is critical for tolerance. Systemic challenge with apoptotic cells induced rapid production of CCL22 in CD169+ (metallophillic) macrophages, resulting in accumulation and activation of FoxP3+ Tregs and CD11c+ dendritic cells, an effect that could be inhibited by antagonizing CCL22-driven chemotaxis. This mechanism was essential for suppression after apoptotic cell challenge, because neutralizing CCL22 or its receptor, reducing Treg numbers, or blocking effector mechanisms abrogated splenic TGF- β and IL-10 induction; this promoted a shift to proinflammatory cytokines associated with a failure to suppress T cells. Similarly, CCR4 inhibition blocked long-term, apoptotic cell-induced tolerance to allografts. Finally, CCR4 inhibition resulted in a systemic breakdown of tolerance to self after apoptotic cell injection with rapid increases in anti-dsDNA IgG and immune complex deposition. Thus, the data demonstrate CCL22-dependent chemotaxis is a key early innate response required for apoptotic cell-induced suppression, implicating a previously unknown mechanism of macrophage-dependent coordination of early events leading to stable tolerance.

Original languageEnglish (US)
Pages (from-to)4215-4220
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number11
DOIs
StatePublished - Mar 18 2014

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Macrophages
Chemotaxis
Innate Immunity
Chemokine CCL22
Transplantation Tolerance
Ego
Antigen-Antibody Complex
Interleukin-10
Dendritic Cells
Apoptosis
Pathology
Cytokines
T-Lymphocytes
Injections
Inhibition (Psychology)

Keywords

  • Autoimmunity
  • Migration
  • Regulation
  • Spleen
  • Transplantation

ASJC Scopus subject areas

  • General

Cite this

Marginal zone CD169+ macrophages coordinate apoptotic cell-driven cellular recruitment and tolerance. / Ravishankar, Buvana; Shinde, Rahul; Liu, Haiyun; Chaudhary, Kapil; Bradley, Jillian; Lemos, Henrique P.; Phillip, Chandlera; Tanaka, Masato; Munn, David H.; Mellor, Andrew L.; McGaha, Tracy L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 11, 18.03.2014, p. 4215-4220.

Research output: Contribution to journalArticle

Ravishankar, B, Shinde, R, Liu, H, Chaudhary, K, Bradley, J, Lemos, HP, Phillip, C, Tanaka, M, Munn, DH, Mellor, AL & McGaha, TL 2014, 'Marginal zone CD169+ macrophages coordinate apoptotic cell-driven cellular recruitment and tolerance', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 11, pp. 4215-4220. https://doi.org/10.1073/pnas.1320924111
Ravishankar, Buvana ; Shinde, Rahul ; Liu, Haiyun ; Chaudhary, Kapil ; Bradley, Jillian ; Lemos, Henrique P. ; Phillip, Chandlera ; Tanaka, Masato ; Munn, David H. ; Mellor, Andrew L. ; McGaha, Tracy L. / Marginal zone CD169+ macrophages coordinate apoptotic cell-driven cellular recruitment and tolerance. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 11. pp. 4215-4220.
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