Maturation of alveolar bone following implantation of an rhGDF-5/PLGA composite into 1-wall intra-bony defects in dogs: 24-week histometric observations

Jung Chul Park, Ulf M E Wikesjö, Ki Tae Koo, Jung Seok Lee, Yong Tae Kim, Susanne D. Pippig, Patrizia Bastone, Chang Sung Kim, Chong Kwan Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective The aim of this study was to evaluate long-term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor-5 (rhGDF-5) in an injectable poly-lactide-co- glycolide-acid (PLGA) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1-wall, critical-size, intra-bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5/PLGA high dose (188 μg/defect) versus sham-surgery control (5 animals), and rhGDF-5/PLGA low dose (37 μg/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24-week healing interval. Results Clinical healing was uneventful. The rhGDF-5 high dose significantly increased bone formation compared with controls in terms of bone area (p < 0.05), and a high degree of bone maturation was observed in the rhGDF-5/PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rhGDF-5/PLGA appears to support alveolar bone healing/regeneration and the rhGDF-5/PLGA high dose uniquely increased maturation of the regenerated bone.

Original languageEnglish (US)
Pages (from-to)565-573
Number of pages9
JournalJournal of Clinical Periodontology
Volume39
Issue number6
DOIs
StatePublished - Jun 1 2012

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Polyglactin 910
Dogs
Bone and Bones
Acids
Root Resorption
Ankylosis
Bone Regeneration
Bicuspid
human GDF5 protein
Jaw
Osteogenesis
Mouth
Tooth
Injections

Keywords

  • PLGA
  • dog
  • periodontal regeneration
  • rhGDF-5
  • tissue engineering

ASJC Scopus subject areas

  • Periodontics

Cite this

Maturation of alveolar bone following implantation of an rhGDF-5/PLGA composite into 1-wall intra-bony defects in dogs : 24-week histometric observations. / Park, Jung Chul; Wikesjö, Ulf M E; Koo, Ki Tae; Lee, Jung Seok; Kim, Yong Tae; Pippig, Susanne D.; Bastone, Patrizia; Kim, Chang Sung; Kim, Chong Kwan.

In: Journal of Clinical Periodontology, Vol. 39, No. 6, 01.06.2012, p. 565-573.

Research output: Contribution to journalArticle

Park, Jung Chul ; Wikesjö, Ulf M E ; Koo, Ki Tae ; Lee, Jung Seok ; Kim, Yong Tae ; Pippig, Susanne D. ; Bastone, Patrizia ; Kim, Chang Sung ; Kim, Chong Kwan. / Maturation of alveolar bone following implantation of an rhGDF-5/PLGA composite into 1-wall intra-bony defects in dogs : 24-week histometric observations. In: Journal of Clinical Periodontology. 2012 ; Vol. 39, No. 6. pp. 565-573.
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abstract = "Objective The aim of this study was to evaluate long-term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor-5 (rhGDF-5) in an injectable poly-lactide-co- glycolide-acid (PLGA) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1-wall, critical-size, intra-bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5/PLGA high dose (188 μg/defect) versus sham-surgery control (5 animals), and rhGDF-5/PLGA low dose (37 μg/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24-week healing interval. Results Clinical healing was uneventful. The rhGDF-5 high dose significantly increased bone formation compared with controls in terms of bone area (p < 0.05), and a high degree of bone maturation was observed in the rhGDF-5/PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rhGDF-5/PLGA appears to support alveolar bone healing/regeneration and the rhGDF-5/PLGA high dose uniquely increased maturation of the regenerated bone.",
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T2 - 24-week histometric observations

AU - Park, Jung Chul

AU - Wikesjö, Ulf M E

AU - Koo, Ki Tae

AU - Lee, Jung Seok

AU - Kim, Yong Tae

AU - Pippig, Susanne D.

AU - Bastone, Patrizia

AU - Kim, Chang Sung

AU - Kim, Chong Kwan

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N2 - Objective The aim of this study was to evaluate long-term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor-5 (rhGDF-5) in an injectable poly-lactide-co- glycolide-acid (PLGA) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1-wall, critical-size, intra-bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5/PLGA high dose (188 μg/defect) versus sham-surgery control (5 animals), and rhGDF-5/PLGA low dose (37 μg/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24-week healing interval. Results Clinical healing was uneventful. The rhGDF-5 high dose significantly increased bone formation compared with controls in terms of bone area (p < 0.05), and a high degree of bone maturation was observed in the rhGDF-5/PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rhGDF-5/PLGA appears to support alveolar bone healing/regeneration and the rhGDF-5/PLGA high dose uniquely increased maturation of the regenerated bone.

AB - Objective The aim of this study was to evaluate long-term (24 weeks) alveolar bone maturation following surgical application of recombinant human growth/differentiation factor-5 (rhGDF-5) in an injectable poly-lactide-co- glycolide-acid (PLGA) composite carrier using an established periodontal defect model. Methods Routine, bilateral, 4 × 5 mm (width × depth), 1-wall, critical-size, intra-bony periodontal defects were surgically created at the 2nd and 4th mandibular premolar teeth in 10 Beagle dogs. The animals were randomized to receive (split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5/PLGA high dose (188 μg/defect) versus sham-surgery control (5 animals), and rhGDF-5/PLGA low dose (37 μg/defect) versus carrier control (5 animals). The animals were euthanized for histometric analysis following a 24-week healing interval. Results Clinical healing was uneventful. The rhGDF-5 high dose significantly increased bone formation compared with controls in terms of bone area (p < 0.05), and a high degree of bone maturation was observed in the rhGDF-5/PLGA high dose group. Root resorption/ankylosis or other aberrant healing events were not observed. Conclusion The rhGDF-5/PLGA appears to support alveolar bone healing/regeneration and the rhGDF-5/PLGA high dose uniquely increased maturation of the regenerated bone.

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