Maturation of antigen-presenting cells is compromised in HLA-G transgenic mice

Anatolij Horuzsko, Francoise Lenfant, David H. Munn, Andrew L. Mellor

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The human MHC class lb antigen HLA-G is thought to regulate maternal immune responses during pregnancy. Here we show that expression of HLA-G in transgenic mice diminished cellular immunity by inhibiting maturation of myelomonocytic cells into functional antigen-presenting cells (APC). Skin allografts applied to HLA-G transgenic mice survived longer and resultant T cell responses were less potent compared to control mice. T cells from HLA-G mice responded normally to allogeneic APC and immunohistological analyses of spleen revealed no marked abnormalities. However, spontaneous outgrowths of myeloid cells were observed when bone marrow or splenocytes from HLA-G mice were cultured in vitro, but functionally competent APC did not develop spontaneously in bone marrow cultures supplemented with granulocyte macrophage colony stimulating factor (GM-CSF). Addition of lipopolysaccharide (LPS) to GM-CSF-derived bone marrow cultures rescued APC maturation. Studies using HLA-G tetrameric reagents revealed that HLA-G-specific binding activity was associated with CD11c+ myelomonocytic cells, while binding to lymphoid and NK cell subsets was undetectable. These data show that spontaneous maturation of functionally competent dendritic cells (DC) is compromised in HLA-G mice. We hypothesize that HLA-G inhibits maturation of DC via receptor-mediated interactions with myelomonocytic precursors, which render immature DC precursors unable to receive signals from activated T cells.

Original languageEnglish (US)
Pages (from-to)385-394
Number of pages10
JournalInternational Immunology
Volume13
Issue number3
DOIs
StatePublished - Jan 1 2001

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HLA-G Antigens
Antigen-Presenting Cells
Transgenic Mice
Dendritic Cells
Bone Marrow
Granulocyte-Macrophage Colony-Stimulating Factor
T-Lymphocytes
Myeloid Cells
Cellular Immunity
Natural Killer Cells
Allografts
Lipopolysaccharides
Spleen
Mothers
Lymphocytes

Keywords

  • Dendritic cells
  • HLA-G
  • Mice
  • T cells

ASJC Scopus subject areas

  • Immunology

Cite this

Maturation of antigen-presenting cells is compromised in HLA-G transgenic mice. / Horuzsko, Anatolij; Lenfant, Francoise; Munn, David H.; Mellor, Andrew L.

In: International Immunology, Vol. 13, No. 3, 01.01.2001, p. 385-394.

Research output: Contribution to journalArticle

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