Maturation of periodontal tissues following implantation of rhGDF-5/β-TCP in one-wall intra-bony defects in dogs: 24-week histological observations

Jung Seok Lee, Ulf M E Wikesjö, Jung Chul Park, Yong Ju Jang, Susanne D. Pippig, Patrizia Bastone, Seong Ho Choi, Chong Kwan Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective Although a previous study reported that recombinant human growth/differentiation factor-5 (rhGDF-5) coated onto a β- tricalciumphosphate (β-TCP) significantly enhanced periodontal regeneration, the long-term stability/maturation of the regenerated tissues has not been demonstrated. The objective of this study was to evaluate periodontal regeneration/maturation following application of rhGDF-5/β-TCP using an established periodontal defect model and a 24-week healing interval. Material & Methods Unilateral, surgically created, 4 à - 4 à - 5 mm (length à - width à - height), one-wall, critical-size, intra-bony periodontal defects at the mandibular second and fourth premolar teeth in five young adult Beagle dogs received rhGDF-5/β-TCP. Bilateral sites at the fourth premolar in the other four dogs served as pristine controls receiving mucogingival flap surgery without defect induction. The animals were euthanized at 24 weeks for histological analysis. Unpublished data from the previous 8-week study were used to compare tissue maturation between 8 and 24 weeks. Results Linear histometric observations of cementum and alveolar regeneration showed no significant differences between the 8- and 24-week observation intervals. However, parameters of periodontal tissue maturation showed significant differences between the observation intervals including increased fraction mineralized tissue and lamellar bone (p < 0.05) and decreased osteocyte counts (p < 0.05) at 24 weeks compared with 8 weeks. Although the count inserting Sharpey's fibre did not significantly change, regenerated cementum remote from the intact periodontal ligament appeared more highly mineralized and thicker at 24 weeks compared with 8 weeks, and compared with the pristine cementum. Minimal β-TCP remained. Conclusions These 24-week observations suggest that regenerated periodontal tissues in sites receiving rhGDF-5/β-TCP undergo progressive maturation without debilitating aberrant tissue reactions.

Original languageEnglish (US)
Pages (from-to)466-474
Number of pages9
JournalJournal of Clinical Periodontology
Volume39
Issue number5
DOIs
StatePublished - May 1 2012

Fingerprint

Dental Cementum
Dogs
Regeneration
Bicuspid
Observation
Osteocytes
Choristoma
Periodontal Ligament
Young Adult
Tooth
Bone and Bones
human GDF5 protein

Keywords

  • 24 weeks
  • dog
  • maturation
  • periodontal regeneration
  • rhGDF-5
  • tissue engineering

ASJC Scopus subject areas

  • Periodontics

Cite this

Maturation of periodontal tissues following implantation of rhGDF-5/β-TCP in one-wall intra-bony defects in dogs : 24-week histological observations. / Lee, Jung Seok; Wikesjö, Ulf M E; Park, Jung Chul; Jang, Yong Ju; Pippig, Susanne D.; Bastone, Patrizia; Choi, Seong Ho; Kim, Chong Kwan.

In: Journal of Clinical Periodontology, Vol. 39, No. 5, 01.05.2012, p. 466-474.

Research output: Contribution to journalArticle

Lee, Jung Seok ; Wikesjö, Ulf M E ; Park, Jung Chul ; Jang, Yong Ju ; Pippig, Susanne D. ; Bastone, Patrizia ; Choi, Seong Ho ; Kim, Chong Kwan. / Maturation of periodontal tissues following implantation of rhGDF-5/β-TCP in one-wall intra-bony defects in dogs : 24-week histological observations. In: Journal of Clinical Periodontology. 2012 ; Vol. 39, No. 5. pp. 466-474.
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abstract = "Objective Although a previous study reported that recombinant human growth/differentiation factor-5 (rhGDF-5) coated onto a β- tricalciumphosphate (β-TCP) significantly enhanced periodontal regeneration, the long-term stability/maturation of the regenerated tissues has not been demonstrated. The objective of this study was to evaluate periodontal regeneration/maturation following application of rhGDF-5/β-TCP using an established periodontal defect model and a 24-week healing interval. Material & Methods Unilateral, surgically created, 4 {\~A} - 4 {\~A} - 5 mm (length {\~A} - width {\~A} - height), one-wall, critical-size, intra-bony periodontal defects at the mandibular second and fourth premolar teeth in five young adult Beagle dogs received rhGDF-5/β-TCP. Bilateral sites at the fourth premolar in the other four dogs served as pristine controls receiving mucogingival flap surgery without defect induction. The animals were euthanized at 24 weeks for histological analysis. Unpublished data from the previous 8-week study were used to compare tissue maturation between 8 and 24 weeks. Results Linear histometric observations of cementum and alveolar regeneration showed no significant differences between the 8- and 24-week observation intervals. However, parameters of periodontal tissue maturation showed significant differences between the observation intervals including increased fraction mineralized tissue and lamellar bone (p < 0.05) and decreased osteocyte counts (p < 0.05) at 24 weeks compared with 8 weeks. Although the count inserting Sharpey's fibre did not significantly change, regenerated cementum remote from the intact periodontal ligament appeared more highly mineralized and thicker at 24 weeks compared with 8 weeks, and compared with the pristine cementum. Minimal β-TCP remained. Conclusions These 24-week observations suggest that regenerated periodontal tissues in sites receiving rhGDF-5/β-TCP undergo progressive maturation without debilitating aberrant tissue reactions.",
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T1 - Maturation of periodontal tissues following implantation of rhGDF-5/β-TCP in one-wall intra-bony defects in dogs

T2 - 24-week histological observations

AU - Lee, Jung Seok

AU - Wikesjö, Ulf M E

AU - Park, Jung Chul

AU - Jang, Yong Ju

AU - Pippig, Susanne D.

AU - Bastone, Patrizia

AU - Choi, Seong Ho

AU - Kim, Chong Kwan

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N2 - Objective Although a previous study reported that recombinant human growth/differentiation factor-5 (rhGDF-5) coated onto a β- tricalciumphosphate (β-TCP) significantly enhanced periodontal regeneration, the long-term stability/maturation of the regenerated tissues has not been demonstrated. The objective of this study was to evaluate periodontal regeneration/maturation following application of rhGDF-5/β-TCP using an established periodontal defect model and a 24-week healing interval. Material & Methods Unilateral, surgically created, 4 à - 4 à - 5 mm (length à - width à - height), one-wall, critical-size, intra-bony periodontal defects at the mandibular second and fourth premolar teeth in five young adult Beagle dogs received rhGDF-5/β-TCP. Bilateral sites at the fourth premolar in the other four dogs served as pristine controls receiving mucogingival flap surgery without defect induction. The animals were euthanized at 24 weeks for histological analysis. Unpublished data from the previous 8-week study were used to compare tissue maturation between 8 and 24 weeks. Results Linear histometric observations of cementum and alveolar regeneration showed no significant differences between the 8- and 24-week observation intervals. However, parameters of periodontal tissue maturation showed significant differences between the observation intervals including increased fraction mineralized tissue and lamellar bone (p < 0.05) and decreased osteocyte counts (p < 0.05) at 24 weeks compared with 8 weeks. Although the count inserting Sharpey's fibre did not significantly change, regenerated cementum remote from the intact periodontal ligament appeared more highly mineralized and thicker at 24 weeks compared with 8 weeks, and compared with the pristine cementum. Minimal β-TCP remained. Conclusions These 24-week observations suggest that regenerated periodontal tissues in sites receiving rhGDF-5/β-TCP undergo progressive maturation without debilitating aberrant tissue reactions.

AB - Objective Although a previous study reported that recombinant human growth/differentiation factor-5 (rhGDF-5) coated onto a β- tricalciumphosphate (β-TCP) significantly enhanced periodontal regeneration, the long-term stability/maturation of the regenerated tissues has not been demonstrated. The objective of this study was to evaluate periodontal regeneration/maturation following application of rhGDF-5/β-TCP using an established periodontal defect model and a 24-week healing interval. Material & Methods Unilateral, surgically created, 4 à - 4 à - 5 mm (length à - width à - height), one-wall, critical-size, intra-bony periodontal defects at the mandibular second and fourth premolar teeth in five young adult Beagle dogs received rhGDF-5/β-TCP. Bilateral sites at the fourth premolar in the other four dogs served as pristine controls receiving mucogingival flap surgery without defect induction. The animals were euthanized at 24 weeks for histological analysis. Unpublished data from the previous 8-week study were used to compare tissue maturation between 8 and 24 weeks. Results Linear histometric observations of cementum and alveolar regeneration showed no significant differences between the 8- and 24-week observation intervals. However, parameters of periodontal tissue maturation showed significant differences between the observation intervals including increased fraction mineralized tissue and lamellar bone (p < 0.05) and decreased osteocyte counts (p < 0.05) at 24 weeks compared with 8 weeks. Although the count inserting Sharpey's fibre did not significantly change, regenerated cementum remote from the intact periodontal ligament appeared more highly mineralized and thicker at 24 weeks compared with 8 weeks, and compared with the pristine cementum. Minimal β-TCP remained. Conclusions These 24-week observations suggest that regenerated periodontal tissues in sites receiving rhGDF-5/β-TCP undergo progressive maturation without debilitating aberrant tissue reactions.

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