Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis

In situ, in vivo, and in vitro studies

R. Jotwani, A. K. Palucka, M. Al-Quotub, M. Nouri-Shirazi, J. Kim, D. Bell, J. Banchereau, Christopher W Cutler

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Previous studies have analyzed the lymphoid and myeloid foci within the gingival mucosa in health and chronic periodontitis (CP); however, the principal APCs responsible for the formation and organizational structure of these foci in CP have not been defined. We show that in human CP tissues, CD1a+ immature Langerhans cells predominantly infiltrate the gingival epithelium, whereas CD83+ mature dendritic cells (DCs) specifically infiltrate the CD4+ lymphoid-rich lamina propria. In vivo evidence shows that exacerbation of CP results in increased levels of proinflammatory cytokines that mediate DC activation/maturation, but also of counterregulatory cytokines that may prevent a Th-polarized response. Consistently, in vitro-generated monocyte-derived DCs pulsed with Porphyromonas gingivalis strain 381 or its LPS undergo maturation, up-regulate accessory molecules, and release proinflammatory (IL-1β, PGE2) and Th (IL-10, IL-12) cytokines. Interestingly, the IL-10:IL-12 ratio elicited from P. gingivalis-pulsed DCs was 3-fold higher than that from Escherichia coli-pulsed DCs. This may account for the significantly (p < 0.05) lower proliferation of autologous CD4+ T cells and reduced release of IFN-γ elicited by P. gingivalis-pulsed DCs. Taken together, these findings suggest a previously unreported mechanism for the pathophysiology of CP, involving the activation and in situ maturation of DCs by the oral pathogen P. gingivalis, leading to release of counterregulatory cytokines and the formation of T cell-DC foci.

Original languageEnglish (US)
Pages (from-to)4693-4700
Number of pages8
JournalJournal of Immunology
Volume167
Issue number8
DOIs
StatePublished - Oct 15 2001
Externally publishedYes

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Chronic Periodontitis
Mouth Mucosa
Dendritic Cells
T-Lymphocytes
Porphyromonas gingivalis
Cytokines
Interleukin-12
Interleukin-10
Mucous Membrane
Langerhans Cells
In Vitro Techniques
Interleukin-1
Dinoprostone
Monocytes
Up-Regulation
Epithelium
Escherichia coli
Health

ASJC Scopus subject areas

  • Immunology

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Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis : In situ, in vivo, and in vitro studies. / Jotwani, R.; Palucka, A. K.; Al-Quotub, M.; Nouri-Shirazi, M.; Kim, J.; Bell, D.; Banchereau, J.; Cutler, Christopher W.

In: Journal of Immunology, Vol. 167, No. 8, 15.10.2001, p. 4693-4700.

Research output: Contribution to journalArticle

Jotwani, R. ; Palucka, A. K. ; Al-Quotub, M. ; Nouri-Shirazi, M. ; Kim, J. ; Bell, D. ; Banchereau, J. ; Cutler, Christopher W. / Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis : In situ, in vivo, and in vitro studies. In: Journal of Immunology. 2001 ; Vol. 167, No. 8. pp. 4693-4700.
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