McN-A-343, a specific agonist of M1-muscarinic receptors, exerts antinicotinic and antimuscarinic effects in the rat adrenal medulla

Arun R. Wakade, Richard Kahn, Ravindra K. Malhotra, Chandramohan G. Wakade, Taruna D. Wakade

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pirenzepine, McN-A-343 and oxotremorine were used to determine the subtypes of muscarinic receptors involved in the secretion of catecholamines from the isolated perfused adrenal gland of the rat. In the presence of 0.1 μM pirenzepine, the concentration-secretion curve for muscarie was shifted in parallel to the right by almost one log unit. With 0.5 μM the shift was over two log units. The apparent dissociation constant for pirenzepine was about 1.12 × 10-8 M. Perfusion with McN-A-343 (1-30 μM) did not evoke the secretion of catecholamines. A further increase to very high concentrations (100-1000 μM) caused only a modest secretion (about 50ng/5 min with 300 μM as compared to the same amount of secretion obatined with 1 μM muscarine). Secretion evoked by nicotine was significantly reduced (30%) by 3 μM McN-A-343, and the inhibition increased (90%) with higher concentrations (100 μM). McN-A-343 also produced concentration-dependent inhibition of catecholamine secretion evoked by muscarine. A significant effect was observed at 30 μM and reached a maximum level at 300 μM. Oxotremorine, like McN-A-343 was a partial agonist on the muscarinic receptors; but unlike McN-A-343, did not block the stimulatory effects of nicotine. Although the pirenzepine data suggest that M1 receptors are responsible for the secretion of catecholamines in the rat adrenal medulla, this conclusion is not supported by the results obtained with the M1-receptor agonist, McN-A-343, which proved to be an effective blocker of muscarinic as well as nicotinic receptors.

Original languageEnglish (US)
Pages (from-to)2073-2080
Number of pages8
JournalLife Sciences
Volume39
Issue number22
DOIs
StatePublished - Dec 1 1986
Externally publishedYes

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(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride
Muscarinic M1 Receptors
Muscarinic Antagonists
Adrenal Medulla
Rats
Pirenzepine
Catecholamines
Muscarinic Receptors
Muscarine
Nicotine
Oxotremorine
Nicotinic Receptors
Adrenal Glands
Cholinergic Agents
Perfusion

ASJC Scopus subject areas

  • Pharmacology

Cite this

McN-A-343, a specific agonist of M1-muscarinic receptors, exerts antinicotinic and antimuscarinic effects in the rat adrenal medulla. / Wakade, Arun R.; Kahn, Richard; Malhotra, Ravindra K.; Wakade, Chandramohan G.; Wakade, Taruna D.

In: Life Sciences, Vol. 39, No. 22, 01.12.1986, p. 2073-2080.

Research output: Contribution to journalArticle

Wakade, Arun R. ; Kahn, Richard ; Malhotra, Ravindra K. ; Wakade, Chandramohan G. ; Wakade, Taruna D. / McN-A-343, a specific agonist of M1-muscarinic receptors, exerts antinicotinic and antimuscarinic effects in the rat adrenal medulla. In: Life Sciences. 1986 ; Vol. 39, No. 22. pp. 2073-2080.
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abstract = "Pirenzepine, McN-A-343 and oxotremorine were used to determine the subtypes of muscarinic receptors involved in the secretion of catecholamines from the isolated perfused adrenal gland of the rat. In the presence of 0.1 μM pirenzepine, the concentration-secretion curve for muscarie was shifted in parallel to the right by almost one log unit. With 0.5 μM the shift was over two log units. The apparent dissociation constant for pirenzepine was about 1.12 × 10-8 M. Perfusion with McN-A-343 (1-30 μM) did not evoke the secretion of catecholamines. A further increase to very high concentrations (100-1000 μM) caused only a modest secretion (about 50ng/5 min with 300 μM as compared to the same amount of secretion obatined with 1 μM muscarine). Secretion evoked by nicotine was significantly reduced (30{\%}) by 3 μM McN-A-343, and the inhibition increased (90{\%}) with higher concentrations (100 μM). McN-A-343 also produced concentration-dependent inhibition of catecholamine secretion evoked by muscarine. A significant effect was observed at 30 μM and reached a maximum level at 300 μM. Oxotremorine, like McN-A-343 was a partial agonist on the muscarinic receptors; but unlike McN-A-343, did not block the stimulatory effects of nicotine. Although the pirenzepine data suggest that M1 receptors are responsible for the secretion of catecholamines in the rat adrenal medulla, this conclusion is not supported by the results obtained with the M1-receptor agonist, McN-A-343, which proved to be an effective blocker of muscarinic as well as nicotinic receptors.",
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AB - Pirenzepine, McN-A-343 and oxotremorine were used to determine the subtypes of muscarinic receptors involved in the secretion of catecholamines from the isolated perfused adrenal gland of the rat. In the presence of 0.1 μM pirenzepine, the concentration-secretion curve for muscarie was shifted in parallel to the right by almost one log unit. With 0.5 μM the shift was over two log units. The apparent dissociation constant for pirenzepine was about 1.12 × 10-8 M. Perfusion with McN-A-343 (1-30 μM) did not evoke the secretion of catecholamines. A further increase to very high concentrations (100-1000 μM) caused only a modest secretion (about 50ng/5 min with 300 μM as compared to the same amount of secretion obatined with 1 μM muscarine). Secretion evoked by nicotine was significantly reduced (30%) by 3 μM McN-A-343, and the inhibition increased (90%) with higher concentrations (100 μM). McN-A-343 also produced concentration-dependent inhibition of catecholamine secretion evoked by muscarine. A significant effect was observed at 30 μM and reached a maximum level at 300 μM. Oxotremorine, like McN-A-343 was a partial agonist on the muscarinic receptors; but unlike McN-A-343, did not block the stimulatory effects of nicotine. Although the pirenzepine data suggest that M1 receptors are responsible for the secretion of catecholamines in the rat adrenal medulla, this conclusion is not supported by the results obtained with the M1-receptor agonist, McN-A-343, which proved to be an effective blocker of muscarinic as well as nicotinic receptors.

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