Measurement of intestinal vascular capacitance in dogs: An application of blood pool scintigraphy

N. W. Scott-Douglas, D. E. Manyari, O. A. Smiseth, Vincent Jb Robinson, S. Y. Wang, E. R. Smith, J. V. Tyberg

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To define relative changes in intestinal vascular capacitance, we developed a model that allowed us to construct intestinal vascular pressure- volume relationships (PVR). Thirteen α-chloralose-anesthetized and splenectomized dogs were studied using a pneumatic constrictor and a small catheter to change and measure portal venous pressure. A small lead sheet was placed beneath the abdominal wall. Relative changes in intestinal blood volume (IBV) were determined by in vivo blood pool scintigraphy with 99mTc-labeled erythrocytes and were expressed as percentages corrected for specific activity and abdominal wall radioactivity. PVRs were constructed using data recorded during graded inflations of the portal venous constrictor. The abdominal wall contributed 32.4 ± 7.7% (SD) of the total counts. During a 4-h control period, PVRs varied by no more than 6% (of IBV). In the isolated intestinal circulation, the change in IBV was precisely proportional to the volume of blood added, indicating that this method can detect very small changes in volume (≤5 ml). Nitroglycerin (25 μg · kg- 1 · min-1) increased capacitance by 20%. Although it measures only relative changes, the model is stable and sensitive, provides reproducible measurement of intestinal PVRs, and, with adaptation, may prove useful in patient studies.

Original languageEnglish (US)
Pages (from-to)232-238
Number of pages7
JournalJournal of Applied Physiology
Volume78
Issue number1
DOIs
StatePublished - Jan 1 1995

Fingerprint

Vascular Capacitance
Gated Blood-Pool Imaging
Blood Volume
Abdominal Wall
Dogs
Portal Pressure
Chloralose
Economic Inflation
Nitroglycerin
Radioactivity
Blood Vessels
Catheters
Erythrocytes
Pressure

Keywords

  • blood volume
  • intestinal blood volume
  • vascular pressure-volume relationships

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Scott-Douglas, N. W., Manyari, D. E., Smiseth, O. A., Robinson, V. J., Wang, S. Y., Smith, E. R., & Tyberg, J. V. (1995). Measurement of intestinal vascular capacitance in dogs: An application of blood pool scintigraphy. Journal of Applied Physiology, 78(1), 232-238. https://doi.org/10.1152/jappl.1995.78.1.232

Measurement of intestinal vascular capacitance in dogs : An application of blood pool scintigraphy. / Scott-Douglas, N. W.; Manyari, D. E.; Smiseth, O. A.; Robinson, Vincent Jb; Wang, S. Y.; Smith, E. R.; Tyberg, J. V.

In: Journal of Applied Physiology, Vol. 78, No. 1, 01.01.1995, p. 232-238.

Research output: Contribution to journalArticle

Scott-Douglas, N. W. ; Manyari, D. E. ; Smiseth, O. A. ; Robinson, Vincent Jb ; Wang, S. Y. ; Smith, E. R. ; Tyberg, J. V. / Measurement of intestinal vascular capacitance in dogs : An application of blood pool scintigraphy. In: Journal of Applied Physiology. 1995 ; Vol. 78, No. 1. pp. 232-238.
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