Measurement of renal tissue oxygen tension

Systematic differences between fluorescence optode and microelectrode recordings in anaesthetized rabbits

Chai Ling Leong, Paul M O'Connor, Gabriela A. Eppel, Warwick P. Anderson, Roger G. Evans

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background/Aims: The validity of fluorescence optodes for measurement of renal cortical tissue oxygen tension was tested by comparison with Clark electrodes. Methods: We varied renal blood flow and inspired O2 content in anaesthetized rabbits while simultaneously measuring cortical tissue oxygen tension. Results: Cortical oxygen tension varied with inspired O 2 content. Fluorescence optode measurements were more tightly distributed than those from a Clark electrode. Cumulative frequency distributions for fluorescence optodes were shifted to the left of those for Clark electrodes. The slope of the relationship between oxygen tension in arterial blood and cortical tissue was less for the fluorescence optode than the Clark electrode. Cortical tissue oxygen tension measurements by these two methods were correlated (r2 = 0.32; p < 0.001), with no fixed bias but considerable proportional bias. Thus, the slope of the relationship between the two measurements was less than unity (0.57 [0.50-0.69]). Conclusion: Cortical oxygen tension values from fluorescence optodes are less variable but proportionally less than those from Clark electrodes. Theoretical considerations suggest that true interstitial oxygen tension lies somewhere between values provided by the two techniques. Nevertheless, the lesser variability of the fluorescence optode technique may aid detection of physiologically significant changes in intrarenal oxygenation.

Original languageEnglish (US)
JournalNephron - Physiology
Volume108
Issue number2
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

Fingerprint

Microelectrodes
Fluorescence
Oxygen
Rabbits
Kidney
Electrodes
Renal Circulation
Arterial Pressure

Keywords

  • Clark electrode
  • Fluorescence quenching
  • Hyperoxia
  • Hypoxia
  • Kidney circulation
  • Renal oxygenation
  • Tissue oxygen tension

ASJC Scopus subject areas

  • Physiology
  • Nephrology
  • Physiology (medical)

Cite this

Measurement of renal tissue oxygen tension : Systematic differences between fluorescence optode and microelectrode recordings in anaesthetized rabbits. / Leong, Chai Ling; O'Connor, Paul M; Eppel, Gabriela A.; Anderson, Warwick P.; Evans, Roger G.

In: Nephron - Physiology, Vol. 108, No. 2, 01.03.2008.

Research output: Contribution to journalArticle

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