Measurement of thromboxane and prostacyclin in valvulotomized human saphenous veins

Scott A. Barman, Saqib S. Chaudhry, Ahamed S. Moideen, John Iljas, A. Addison Barman

Research output: Contribution to journalArticle

Abstract

The present study was done to determine the effect of the modified Hall valvulotome technique on endothelial injury by measuring TxB2 and 6-keto PGF(1a), the stable metabolites of thromboxane and prostacyclin, respectively. It was hypothesized that increased levels of these cyclooxygenase products would be an excellent indicator of vascular endothelial injury in the presence of the modified Hall valvulotome. Eight segments of human distal saphenous veins were obtained, each measuring approximately 4 cm in length, with diameters of approximately 2 to 3 mm. From these original vein segments, two groups of smaller vein segments were examined, with each group consisting of eight segments, each segment measuring 2 cm in length. The first group of vein segments was designated as the control group, and the second group of vessels had a modified Hall valvulotome (2.5 mm size) inserted into each segment to simulate valvulotomy. After this procedure, all vein segments were analyzed for levels of thromboxane and prostacyclin by a standard radioimmunoassay procedure. Results from the present study indicate that the modified Hall valvulotome technique in human saphenous veins does not significantly increase the levels of the cyclooxygenase metabolites thromboxane and prostacyclin relative to control conditions. However, the ratio of TxB2 formation 6-keto PGF(1a) production was increased in the valvulotomized vessel segments, indicating possible platelet release of thromboxane. Therefore, even though there was increased thromboxane production relative to prostacyclin levels in the modified Hall valvulotome technique, it still appears that this type of valvulotomy is relatively noninsulting to the endothelial cell lining.

Original languageEnglish (US)
Pages (from-to)291-293
Number of pages3
JournalAmerican Surgeon
Volume63
Issue number3
StatePublished - Apr 7 1997

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Thromboxanes
Saphenous Vein
Epoprostenol
Veins
Prostaglandins F
Prostaglandin-Endoperoxide Synthases
Vascular System Injuries
Radioimmunoassay
Blood Platelets
Endothelial Cells
Control Groups
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Barman, S. A., Chaudhry, S. S., Moideen, A. S., Iljas, J., & Barman, A. A. (1997). Measurement of thromboxane and prostacyclin in valvulotomized human saphenous veins. American Surgeon, 63(3), 291-293.

Measurement of thromboxane and prostacyclin in valvulotomized human saphenous veins. / Barman, Scott A.; Chaudhry, Saqib S.; Moideen, Ahamed S.; Iljas, John; Barman, A. Addison.

In: American Surgeon, Vol. 63, No. 3, 07.04.1997, p. 291-293.

Research output: Contribution to journalArticle

Barman, SA, Chaudhry, SS, Moideen, AS, Iljas, J & Barman, AA 1997, 'Measurement of thromboxane and prostacyclin in valvulotomized human saphenous veins', American Surgeon, vol. 63, no. 3, pp. 291-293.
Barman SA, Chaudhry SS, Moideen AS, Iljas J, Barman AA. Measurement of thromboxane and prostacyclin in valvulotomized human saphenous veins. American Surgeon. 1997 Apr 7;63(3):291-293.
Barman, Scott A. ; Chaudhry, Saqib S. ; Moideen, Ahamed S. ; Iljas, John ; Barman, A. Addison. / Measurement of thromboxane and prostacyclin in valvulotomized human saphenous veins. In: American Surgeon. 1997 ; Vol. 63, No. 3. pp. 291-293.
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