Measuring circulating cytokines

Joseph Gerard Cannon, J. L. Nerad, D. D. Poutsiaka, C. A. Dinarello

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Cytokines, i.e., regulatory proteins derived primarily (but not exclusively) from cells of the immune system, are receiving increasing attention for their influences on physiological processes. This paper outlines several of the unique characteristics of cytokines and discusses the pitfalls encountered when measuring them in biological fluids. At present, each available assay has a combination of advantages and drawbacks; therefore, investigators must be aware of the trade-offs and choose the assay that best addresses their needs. The factors that affect cytokine measurement also influence cytokine activity in vivo; thus they are important from a physiological as well as methodological standpoint. Moreover, the absolute concentration of a single cytokine is probably less important than the balance between that cytokine and its natural antagonists.

Original languageEnglish (US)
Pages (from-to)1897-1902
Number of pages6
JournalJournal of Applied Physiology
Volume75
Issue number4
StatePublished - Jan 1 1993

Fingerprint

Cytokines
Physiological Phenomena
Immune System
Research Personnel
Proteins

Keywords

  • bioassay
  • immunoassay
  • interleukin-1
  • plasma
  • serum
  • tumor necrosis factor

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Cannon, J. G., Nerad, J. L., Poutsiaka, D. D., & Dinarello, C. A. (1993). Measuring circulating cytokines. Journal of Applied Physiology, 75(4), 1897-1902.

Measuring circulating cytokines. / Cannon, Joseph Gerard; Nerad, J. L.; Poutsiaka, D. D.; Dinarello, C. A.

In: Journal of Applied Physiology, Vol. 75, No. 4, 01.01.1993, p. 1897-1902.

Research output: Contribution to journalArticle

Cannon, JG, Nerad, JL, Poutsiaka, DD & Dinarello, CA 1993, 'Measuring circulating cytokines', Journal of Applied Physiology, vol. 75, no. 4, pp. 1897-1902.
Cannon JG, Nerad JL, Poutsiaka DD, Dinarello CA. Measuring circulating cytokines. Journal of Applied Physiology. 1993 Jan 1;75(4):1897-1902.
Cannon, Joseph Gerard ; Nerad, J. L. ; Poutsiaka, D. D. ; Dinarello, C. A. / Measuring circulating cytokines. In: Journal of Applied Physiology. 1993 ; Vol. 75, No. 4. pp. 1897-1902.
@article{ad46c44295ac458e90f093ad0126bda9,
title = "Measuring circulating cytokines",
abstract = "Cytokines, i.e., regulatory proteins derived primarily (but not exclusively) from cells of the immune system, are receiving increasing attention for their influences on physiological processes. This paper outlines several of the unique characteristics of cytokines and discusses the pitfalls encountered when measuring them in biological fluids. At present, each available assay has a combination of advantages and drawbacks; therefore, investigators must be aware of the trade-offs and choose the assay that best addresses their needs. The factors that affect cytokine measurement also influence cytokine activity in vivo; thus they are important from a physiological as well as methodological standpoint. Moreover, the absolute concentration of a single cytokine is probably less important than the balance between that cytokine and its natural antagonists.",
keywords = "bioassay, immunoassay, interleukin-1, plasma, serum, tumor necrosis factor",
author = "Cannon, {Joseph Gerard} and Nerad, {J. L.} and Poutsiaka, {D. D.} and Dinarello, {C. A.}",
year = "1993",
month = "1",
day = "1",
language = "English (US)",
volume = "75",
pages = "1897--1902",
journal = "Journal of Applied Physiology Respiratory Environmental and Exercise Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Measuring circulating cytokines

AU - Cannon, Joseph Gerard

AU - Nerad, J. L.

AU - Poutsiaka, D. D.

AU - Dinarello, C. A.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Cytokines, i.e., regulatory proteins derived primarily (but not exclusively) from cells of the immune system, are receiving increasing attention for their influences on physiological processes. This paper outlines several of the unique characteristics of cytokines and discusses the pitfalls encountered when measuring them in biological fluids. At present, each available assay has a combination of advantages and drawbacks; therefore, investigators must be aware of the trade-offs and choose the assay that best addresses their needs. The factors that affect cytokine measurement also influence cytokine activity in vivo; thus they are important from a physiological as well as methodological standpoint. Moreover, the absolute concentration of a single cytokine is probably less important than the balance between that cytokine and its natural antagonists.

AB - Cytokines, i.e., regulatory proteins derived primarily (but not exclusively) from cells of the immune system, are receiving increasing attention for their influences on physiological processes. This paper outlines several of the unique characteristics of cytokines and discusses the pitfalls encountered when measuring them in biological fluids. At present, each available assay has a combination of advantages and drawbacks; therefore, investigators must be aware of the trade-offs and choose the assay that best addresses their needs. The factors that affect cytokine measurement also influence cytokine activity in vivo; thus they are important from a physiological as well as methodological standpoint. Moreover, the absolute concentration of a single cytokine is probably less important than the balance between that cytokine and its natural antagonists.

KW - bioassay

KW - immunoassay

KW - interleukin-1

KW - plasma

KW - serum

KW - tumor necrosis factor

UR - http://www.scopus.com/inward/record.url?scp=0027375799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027375799&partnerID=8YFLogxK

M3 - Article

VL - 75

SP - 1897

EP - 1902

JO - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology

JF - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology

SN - 8750-7587

IS - 4

ER -