Abstract
A method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer.
Original language | English (US) |
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Pages (from-to) | 9-11 |
Number of pages | 3 |
Journal | Analytical Biochemistry |
Volume | 532 |
DOIs | |
State | Published - Sep 1 2017 |
Keywords
- Anisotropy
- Circular dichroism
- Dichrometer
- Linear dichroism
- Photoelastic modulator
- Polarization ratio
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology