Measuring fluorescence polarization with a dichrometer

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer.

Original languageEnglish (US)
Pages (from-to)9-11
Number of pages3
JournalAnalytical Biochemistry
Volume532
DOIs
StatePublished - Sep 1 2017

Keywords

  • Anisotropy
  • Circular dichroism
  • Dichrometer
  • Linear dichroism
  • Photoelastic modulator
  • Polarization ratio

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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