Measuring kidney function in conscious mice.

David L. Mattson

Research output: Contribution to journalReview article

Abstract

Mice lacking or over-expressing a gene of experimental interest have become important tools to understand the regulation of kidney function and water and electrolyte homeostasis. The use of mice in physiological studies is becoming more widespread, but there are still a number of technical limitations that preclude the full utilization of mouse models in renal research. The present chapter focuses upon a set of methods developed in our laboratory to quantify renal function in conscious mice. These measurements are based upon surgical instrumentation of mice with chronic indwelling arterial and venous catheters. This preparation permits direct measurement of arterial blood pressure, direct sampling of arterial and/or venous blood, intravenous or intra-arterial infusion of substances, and quantification of daily sodium balance. The advantage of these techniques is that all of these procedures can be performed in conscious mice freely moving in their home cages. As such, this in vivo preparation provides an assessment of physiological function in mice in their native state.

Original languageEnglish (US)
Pages (from-to)75-94
Number of pages20
JournalMethods in molecular biology (Clifton, N.J.)
Volume573
StatePublished - 2009
Externally publishedYes

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Kidney
Intra Arterial Infusions
Electrolytes
Arterial Pressure
Homeostasis
Catheters
Sodium
Water
Research
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Measuring kidney function in conscious mice. / Mattson, David L.

In: Methods in molecular biology (Clifton, N.J.), Vol. 573, 2009, p. 75-94.

Research output: Contribution to journalReview article

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