Abstract
Objectives: The objective of the present study was to elucidate the mechanism of bioactive molecule extraction from mineralized dentin by calcium hydroxide (Ca(OH)2) and tricalcium silicate cements (TSC). Methods and results: Transmission electron microscopy was used to provide evidence for collagen degradation in dentin surfaces covered with Ca(OH)2 or a set, hydrated TSC for 1–3 months. A one micron thick collagen degradation zone was observed on the dentin surface. Fourier transform-infrared spectroscopy was used to identify increases in apatite/collagen ratio in dentin exposed to Ca(OH)2. Using three-point bending, dentin exposed to Ca(OH)2 exhibited significant reduction in flexural strength. Using size exclusion chromatography, it was found that the small size of the hydroxyl ions derived from Ca(OH)2 enabled those ions to infiltrate the intrafibrillar compartment of mineralized collagen and degrade the collagen fibrils without affecting the apatite minerals. Using ELISA, TGF-β1 was found to be extracted from dentin covered with Ca(OH)2 for 3 months. Unlike acids that dissolve the mineral component of dentin to release bioactive molecules, alkaline materials such as Ca(OH)2 or TSC released growth factors such as TGF-β1 via collagen degradation. Significance: The bioactive molecule extraction capacities of Ca(OH)2 and TSC render these dental materials excellent for pulp capping and endodontic regeneration. These highly desirable properties, however, appear to be intertwined with the untoward effect of degradation of the collagen matrix within mineralized dentin, resulting in reduced flexural strength.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 317-330 |
| Number of pages | 14 |
| Journal | Dental Materials |
| Volume | 34 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2018 |
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Keywords
- Calcium hydroxide
- Collagen degradation
- Dentin
- Flexural strength
- Size congruity
- Tricalcium silicate cement
ASJC Scopus subject areas
- Materials Science(all)
- Dentistry(all)
- Mechanics of Materials
Cite this
Mechanism of bioactive molecular extraction from mineralized dentin by calcium hydroxide and tricalcium silicate cement. / Huang, Xue qing; Camba, John; Gu, Li sha; Bergeron, Brian Edward; Ricucci, Domenico; Pashley, David Henry; Tay, Franklin Chi Meng; Niu, Li na.
In: Dental Materials, Vol. 34, No. 2, 01.02.2018, p. 317-330.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanism of bioactive molecular extraction from mineralized dentin by calcium hydroxide and tricalcium silicate cement
AU - Huang, Xue qing
AU - Camba, John
AU - Gu, Li sha
AU - Bergeron, Brian Edward
AU - Ricucci, Domenico
AU - Pashley, David Henry
AU - Tay, Franklin Chi Meng
AU - Niu, Li na
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Objectives: The objective of the present study was to elucidate the mechanism of bioactive molecule extraction from mineralized dentin by calcium hydroxide (Ca(OH)2) and tricalcium silicate cements (TSC). Methods and results: Transmission electron microscopy was used to provide evidence for collagen degradation in dentin surfaces covered with Ca(OH)2 or a set, hydrated TSC for 1–3 months. A one micron thick collagen degradation zone was observed on the dentin surface. Fourier transform-infrared spectroscopy was used to identify increases in apatite/collagen ratio in dentin exposed to Ca(OH)2. Using three-point bending, dentin exposed to Ca(OH)2 exhibited significant reduction in flexural strength. Using size exclusion chromatography, it was found that the small size of the hydroxyl ions derived from Ca(OH)2 enabled those ions to infiltrate the intrafibrillar compartment of mineralized collagen and degrade the collagen fibrils without affecting the apatite minerals. Using ELISA, TGF-β1 was found to be extracted from dentin covered with Ca(OH)2 for 3 months. Unlike acids that dissolve the mineral component of dentin to release bioactive molecules, alkaline materials such as Ca(OH)2 or TSC released growth factors such as TGF-β1 via collagen degradation. Significance: The bioactive molecule extraction capacities of Ca(OH)2 and TSC render these dental materials excellent for pulp capping and endodontic regeneration. These highly desirable properties, however, appear to be intertwined with the untoward effect of degradation of the collagen matrix within mineralized dentin, resulting in reduced flexural strength.
AB - Objectives: The objective of the present study was to elucidate the mechanism of bioactive molecule extraction from mineralized dentin by calcium hydroxide (Ca(OH)2) and tricalcium silicate cements (TSC). Methods and results: Transmission electron microscopy was used to provide evidence for collagen degradation in dentin surfaces covered with Ca(OH)2 or a set, hydrated TSC for 1–3 months. A one micron thick collagen degradation zone was observed on the dentin surface. Fourier transform-infrared spectroscopy was used to identify increases in apatite/collagen ratio in dentin exposed to Ca(OH)2. Using three-point bending, dentin exposed to Ca(OH)2 exhibited significant reduction in flexural strength. Using size exclusion chromatography, it was found that the small size of the hydroxyl ions derived from Ca(OH)2 enabled those ions to infiltrate the intrafibrillar compartment of mineralized collagen and degrade the collagen fibrils without affecting the apatite minerals. Using ELISA, TGF-β1 was found to be extracted from dentin covered with Ca(OH)2 for 3 months. Unlike acids that dissolve the mineral component of dentin to release bioactive molecules, alkaline materials such as Ca(OH)2 or TSC released growth factors such as TGF-β1 via collagen degradation. Significance: The bioactive molecule extraction capacities of Ca(OH)2 and TSC render these dental materials excellent for pulp capping and endodontic regeneration. These highly desirable properties, however, appear to be intertwined with the untoward effect of degradation of the collagen matrix within mineralized dentin, resulting in reduced flexural strength.
KW - Calcium hydroxide
KW - Collagen degradation
KW - Dentin
KW - Flexural strength
KW - Size congruity
KW - Tricalcium silicate cement
UR - http://www.scopus.com/inward/record.url?scp=85035150623&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85035150623&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2017.11.010
DO - 10.1016/j.dental.2017.11.010
M3 - Article
C2 - 29179973
AN - SCOPUS:85035150623
VL - 34
SP - 317
EP - 330
JO - Dental Materials
JF - Dental Materials
SN - 0109-5641
IS - 2
ER -