Mechanism of differential efficacy of garlic organosulfides in preventing benzo(a)pyrene-induced cancer in mice

Sanjay K. Srivastava, Xun Hu, Hong Xia, Howard A. Zaren, Moushumi L. Chatterjee, Rajesh Agarwal, Shivendra V. Singh

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The mechanism of differential efficacies of diallyl sulfide (DAS), diallyl disulfide (DADS), diallyl trisulfide (DATS), dipropyl sulfide (DPS) and dipropyl disulfide (DPDS) in preventing benzo(a)pyrene (BP)-induced cancer in mice has been investigated by determining their effects on the enzymes of BP activation/inactivation pathways. With the exception of DATS, treatment of mice with other organosulfides (OSCs) caused a small but significant increase (37-44%) in hepatic ethoxyresorufin O-deethylase (EROD) activity. However, the forestomach EROD activity did not differ significantly between control and treated groups. Only DAS treatment caused a modest but statistically significant reduction (about 25%) in pulmonary EROD activity. These results suggest that while reduction of EROD activity may, at least in part, contribute to the DAS-mediated inhibition of BP-induced lung cancer, anticarcinogenic effects of OSCs against BP-induced forestomach carcinogenesis seems to be independent of this mechanism. Treatment of mice with DAS, DADS and DATS resulted in a significant increase, as compared with control, in both hepatic (3.0-, 3.2- and 4.4-fold, respectively) and forestomach (1.5-, 2.7- and 2.7-fold, respectively) glutathione transferase (GST) activity toward anti-7β,8α-dihydroxy-9α,10α-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene (anti-BPDE), which is the ultimate carcinogen of BP. The pulmonary GST activity was not increased by any of the OSCs. Even though epoxide hydrolase (EH) activity was differentially altered by these OSCs, a correlation between chemopreventive efficacy of OSCs and their effects on EH activity was not apparent. The results of the present study suggest that differences in the ability of OSCs to modulate GST activity toward anti-BPDE may, at least in part, account for their differential chemopreventive efficacy against BP-induced cancer in mice.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalCancer Letters
Volume118
Issue number1
DOIs
StatePublished - Sep 16 1997

Fingerprint

Cytochrome P-450 CYP1A1
Garlic
Benzo(a)pyrene
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
Epoxide Hydrolases
Neoplasms
Anticarcinogenic Agents
Lung
Enzyme Activation
Liver
Glutathione Transferase
Carcinogens
Lung Neoplasms
Carcinogenesis
Control Groups
allyl sulfide
diallyl trisulfide
diallyl disulfide

Keywords

  • Benzo(a)pyrene
  • Cancer prevention
  • Carcinogenesis
  • Garlic
  • Glutathione S-transferase
  • Organosulfides

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Srivastava, S. K., Hu, X., Xia, H., Zaren, H. A., Chatterjee, M. L., Agarwal, R., & Singh, S. V. (1997). Mechanism of differential efficacy of garlic organosulfides in preventing benzo(a)pyrene-induced cancer in mice. Cancer Letters, 118(1), 61-67. https://doi.org/10.1016/S0304-3835(97)00237-1

Mechanism of differential efficacy of garlic organosulfides in preventing benzo(a)pyrene-induced cancer in mice. / Srivastava, Sanjay K.; Hu, Xun; Xia, Hong; Zaren, Howard A.; Chatterjee, Moushumi L.; Agarwal, Rajesh; Singh, Shivendra V.

In: Cancer Letters, Vol. 118, No. 1, 16.09.1997, p. 61-67.

Research output: Contribution to journalArticle

Srivastava, SK, Hu, X, Xia, H, Zaren, HA, Chatterjee, ML, Agarwal, R & Singh, SV 1997, 'Mechanism of differential efficacy of garlic organosulfides in preventing benzo(a)pyrene-induced cancer in mice', Cancer Letters, vol. 118, no. 1, pp. 61-67. https://doi.org/10.1016/S0304-3835(97)00237-1
Srivastava, Sanjay K. ; Hu, Xun ; Xia, Hong ; Zaren, Howard A. ; Chatterjee, Moushumi L. ; Agarwal, Rajesh ; Singh, Shivendra V. / Mechanism of differential efficacy of garlic organosulfides in preventing benzo(a)pyrene-induced cancer in mice. In: Cancer Letters. 1997 ; Vol. 118, No. 1. pp. 61-67.
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