MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression

Y. Zha, Y. Xia, J. Ding, Jeong-Hyeon Choi, L. Yang, Zheng Dong, Chunhong Yan, Shuang Huang, Hanfei Ding

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

MEIS2 has an important role in development and organogenesis, and is implicated in the pathogenesis of human cancer. The molecular basis of MEIS2 action in tumorigenesis is not clear. Here, we show that MEIS2 is highly expressed in human neuroblastoma cell lines and is required for neuroblastoma cell survival and proliferation. Depletion of MEIS2 in neuroblastoma cells leads to M-phase arrest and mitotic catastrophe, whereas ectopic expression of MEIS2 markedly enhances neuroblastoma cell proliferation, anchorage-independent growth, and tumorigenicity. Gene expression profiling reveals an essential role of MEIS2 in maintaining the expression of a large number of late cell-cycle genes, including those required for DNA replication, G2-M checkpoint control and M-phase progression. Importantly, we identify MEIS2 as a transcription activator of the MuvB-BMYB-FOXM1 complex that functions as a master regulator of cell-cycle gene expression. Further, we show that FOXM1 is a direct target gene of MEIS2 and is required for MEIS2 to upregulate mitotic genes. These findings link a developmentally important gene to the control of cell proliferation and suggest that high MEIS2 expression is a molecular mechanism for high expression of mitotic genes that is frequently observed in cancers of poor prognosis.

Original languageEnglish (US)
Article numbere1417
JournalCell Death and Disease
Volume5
Issue number9
DOIs
StatePublished - Jan 1 2014

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Neuroblastoma
Cell Division
Cell Survival
Cell Proliferation
cdc Genes
M Phase Cell Cycle Checkpoints
Genes
Gene Expression
Organogenesis
Gene Expression Profiling
DNA Replication
Mitosis
Neoplasms
Carcinogenesis
Up-Regulation
Cell Line
Growth

ASJC Scopus subject areas

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Cite this

MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression. / Zha, Y.; Xia, Y.; Ding, J.; Choi, Jeong-Hyeon; Yang, L.; Dong, Zheng; Yan, Chunhong; Huang, Shuang; Ding, Hanfei.

In: Cell Death and Disease, Vol. 5, No. 9, e1417, 01.01.2014.

Research output: Contribution to journalArticle

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AU - Yang, L.

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