Melanocortin-1 and melanocortin-5 receptors are expressed in cultured mouse peritoneal macrophages

Mitsuyuki Ichinose, Gregg Thomas Nagle, Masatoshi Asai, Masashi Sawada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Adrenocorticotropic hormone (ACTH) belongs to the melanocortin group of related peptides which share a common precursor, pro-opiomelanocortin (POMC). Melanocortin expresses its functional effects by the stimulation of specific seven transmembrane G-protein linked receptors. To date, five melanocortin receptors have been cloned. In the immune system, the production of ACTH in immuno-competent cells has been reported. Because of various physiological effects of ACTH on immuno-competent cells, the hormone might be an intercellular messenger in the immune system. In this study, we report the expression of melanocortin-1 and melanocortin-5 receptors (MC1 and MC5) in cultured mouse peritoneal macrophages. By using a sensitive reverse transcription-polymerase chain reaction (RT-PCR), we demonstrate that these two receptors are specifically expressed in macrophages, whereas expression of other members of the melanocortin receptor family (MC2, MC3 and MC4) was not detected. The sizes of the MC1 and MC5 receptor reverse transcription-polymerase chain reaction (RT-PCR) products are identical to the MC1 receptor RT-PCR product in mouse skin and MC5 receptor RT-PCR product in mouse skeletal muscle. The present data suggest that ACTH may modulate macrophage function by activating MC1 and/or MC5 receptors.

Original languageEnglish (US)
Pages (from-to)169-172
Number of pages4
JournalBiomedical Research
Volume21
Issue number3
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Melanocortins
Macrophages
Polymerase chain reaction
Peritoneal Macrophages
Transcription
Adrenocorticotropic Hormone
Reverse Transcription
Receptor, Melanocortin, Type 1
Reaction products
Melanocortin Receptors
Polymerase Chain Reaction
Immune system
Immune System
Pro-Opiomelanocortin
GTP-Binding Proteins
Muscle
Skin
Skeletal Muscle
Hormones
Peptides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Melanocortin-1 and melanocortin-5 receptors are expressed in cultured mouse peritoneal macrophages. / Ichinose, Mitsuyuki; Nagle, Gregg Thomas; Asai, Masatoshi; Sawada, Masashi.

In: Biomedical Research, Vol. 21, No. 3, 01.01.2000, p. 169-172.

Research output: Contribution to journalArticle

Ichinose, Mitsuyuki ; Nagle, Gregg Thomas ; Asai, Masatoshi ; Sawada, Masashi. / Melanocortin-1 and melanocortin-5 receptors are expressed in cultured mouse peritoneal macrophages. In: Biomedical Research. 2000 ; Vol. 21, No. 3. pp. 169-172.
@article{7ec4ce9d574348b580ca45e92bd3cc95,
title = "Melanocortin-1 and melanocortin-5 receptors are expressed in cultured mouse peritoneal macrophages",
abstract = "Adrenocorticotropic hormone (ACTH) belongs to the melanocortin group of related peptides which share a common precursor, pro-opiomelanocortin (POMC). Melanocortin expresses its functional effects by the stimulation of specific seven transmembrane G-protein linked receptors. To date, five melanocortin receptors have been cloned. In the immune system, the production of ACTH in immuno-competent cells has been reported. Because of various physiological effects of ACTH on immuno-competent cells, the hormone might be an intercellular messenger in the immune system. In this study, we report the expression of melanocortin-1 and melanocortin-5 receptors (MC1 and MC5) in cultured mouse peritoneal macrophages. By using a sensitive reverse transcription-polymerase chain reaction (RT-PCR), we demonstrate that these two receptors are specifically expressed in macrophages, whereas expression of other members of the melanocortin receptor family (MC2, MC3 and MC4) was not detected. The sizes of the MC1 and MC5 receptor reverse transcription-polymerase chain reaction (RT-PCR) products are identical to the MC1 receptor RT-PCR product in mouse skin and MC5 receptor RT-PCR product in mouse skeletal muscle. The present data suggest that ACTH may modulate macrophage function by activating MC1 and/or MC5 receptors.",
author = "Mitsuyuki Ichinose and Nagle, {Gregg Thomas} and Masatoshi Asai and Masashi Sawada",
year = "2000",
month = "1",
day = "1",
doi = "10.2220/biomedres.21.169",
language = "English (US)",
volume = "21",
pages = "169--172",
journal = "Biomedical Research",
issn = "0388-6107",
publisher = "Biomedical Research Foundation",
number = "3",

}

TY - JOUR

T1 - Melanocortin-1 and melanocortin-5 receptors are expressed in cultured mouse peritoneal macrophages

AU - Ichinose, Mitsuyuki

AU - Nagle, Gregg Thomas

AU - Asai, Masatoshi

AU - Sawada, Masashi

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Adrenocorticotropic hormone (ACTH) belongs to the melanocortin group of related peptides which share a common precursor, pro-opiomelanocortin (POMC). Melanocortin expresses its functional effects by the stimulation of specific seven transmembrane G-protein linked receptors. To date, five melanocortin receptors have been cloned. In the immune system, the production of ACTH in immuno-competent cells has been reported. Because of various physiological effects of ACTH on immuno-competent cells, the hormone might be an intercellular messenger in the immune system. In this study, we report the expression of melanocortin-1 and melanocortin-5 receptors (MC1 and MC5) in cultured mouse peritoneal macrophages. By using a sensitive reverse transcription-polymerase chain reaction (RT-PCR), we demonstrate that these two receptors are specifically expressed in macrophages, whereas expression of other members of the melanocortin receptor family (MC2, MC3 and MC4) was not detected. The sizes of the MC1 and MC5 receptor reverse transcription-polymerase chain reaction (RT-PCR) products are identical to the MC1 receptor RT-PCR product in mouse skin and MC5 receptor RT-PCR product in mouse skeletal muscle. The present data suggest that ACTH may modulate macrophage function by activating MC1 and/or MC5 receptors.

AB - Adrenocorticotropic hormone (ACTH) belongs to the melanocortin group of related peptides which share a common precursor, pro-opiomelanocortin (POMC). Melanocortin expresses its functional effects by the stimulation of specific seven transmembrane G-protein linked receptors. To date, five melanocortin receptors have been cloned. In the immune system, the production of ACTH in immuno-competent cells has been reported. Because of various physiological effects of ACTH on immuno-competent cells, the hormone might be an intercellular messenger in the immune system. In this study, we report the expression of melanocortin-1 and melanocortin-5 receptors (MC1 and MC5) in cultured mouse peritoneal macrophages. By using a sensitive reverse transcription-polymerase chain reaction (RT-PCR), we demonstrate that these two receptors are specifically expressed in macrophages, whereas expression of other members of the melanocortin receptor family (MC2, MC3 and MC4) was not detected. The sizes of the MC1 and MC5 receptor reverse transcription-polymerase chain reaction (RT-PCR) products are identical to the MC1 receptor RT-PCR product in mouse skin and MC5 receptor RT-PCR product in mouse skeletal muscle. The present data suggest that ACTH may modulate macrophage function by activating MC1 and/or MC5 receptors.

UR - http://www.scopus.com/inward/record.url?scp=0033778448&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033778448&partnerID=8YFLogxK

U2 - 10.2220/biomedres.21.169

DO - 10.2220/biomedres.21.169

M3 - Article

AN - SCOPUS:0033778448

VL - 21

SP - 169

EP - 172

JO - Biomedical Research

JF - Biomedical Research

SN - 0388-6107

IS - 3

ER -